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Clinical Proteomics

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Antonia Vlahou

    1. Biomedical Research Foundation, Academy of Athens, Athens, Greece

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    You can also search for this editor in PubMed   Google Scholar

  2. Manousos Makridakis

    1. Biomedical Research Foundation, Academy of Athens, Athens, Greece

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1243)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Sample Collection

    1. Front Matter

      Pages 1-1
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    2. Biological Sample Collection for Clinical Proteomics: Existing SOPs

      • Vasiliki Lygirou, Manousos Makridakis, Antonia Vlahou
      Pages 3-27

    3. Targeting the Proteome of Cellular Fractions: Focus on Secreted Proteins

      • Agnieszka Latosinska, Maria Frantzi, William Mullen, Antonia Vlahou, Manousos Makridakis
      Pages 29-41

    4. Preparation of Urinary Exosomes: Methodological Issues for Clinical Proteomics

      • Marina Pitto, Samuele Corbetta, Francesca Raimondo
      Pages 43-53

    5. Sample Treatment Methods Involving Combinatorial Peptide Ligand Libraries for Improved Proteomes Analyses

      • Pier Giorgio Righetti, Egisto Boschetti
      Pages 55-82

    6. Glycoprotein Enrichment Method Using a Selective Magnetic Nano-Probe Platform (MNP) Functionalized with Lectins

      • Marta Cova, Rui Oliveira-Silva, José Alexandre Ferreira, Rita Ferreira, Francisco Amado, Ana Luísa Daniel-da-Silva et al.
      Pages 83-100

  3. Technologies for Discovery and Validation

    1. Front Matter

      Pages 101-101
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    2. The Latest Advancements in Proteomic Two-dimensional Gel Electrophoresis Analysis Applied to Biological Samples

      • Laura Santucci, Maurizio Bruschi, Gian Marco Ghiggeri, Giovanni Candiano
      Pages 103-125

    3. 2DE Maps in the Discovery of Human Autoimmune Kidney Diseases: The Case of Membranous Glomerulonephritis

      • Maurizio Bruschi, Laura Santucci, Gian Marco Ghiggeri, Giovanni Candiano
      Pages 127-138

    4. MALDI-Imaging Mass Spectrometry on Tissues

      • Veronica Mainini, Maciej Lalowski, Athanasios Gotsopoulos, Vasiliki Bitsika, Marc Baumann, Fulvio Magni
      Pages 139-164

    5. Laser Capture Microdissection of Fluorescently Labeled Amyloid Plaques from Alzheimer’s Disease Brain Tissue for Mass Spectrometric Analysis

      • Diana A. T. Nijholt, Christoph Stingl, Theo M. Luider
      Pages 165-173

    6. Urine Sample Preparation and Fractionation for Global Proteome Profiling by LC-MS

      • Magali Court, Jérôme Garin, Christophe D. Masselon
      Pages 175-186

    7. Methods in Capillary Electrophoresis Coupled to Mass Spectrometry for the Identification of Clinical Proteomic/Peptidomic Biomarkers in Biofluids

      • Angelique Stalmach, Holger Husi, Khedidja Mosbahi, Amaya Albalat, William Mullen, Harald Mischak
      Pages 187-205

    8. Quantification of Proteins in Urine Samples Using Targeted Mass Spectrometry Methods

      • Nina Khristenko, Bruno Domon
      Pages 207-220

  4. Extracting Biological Relevant Biomarkers and Proceeding with Implementation

    1. Front Matter

      Pages 221-221
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    2. Statistical Issues in the Design and Planning of Proteomic Profiling Experiments

      • David A. Cairns
      Pages 223-236

    3. Integrating Proteomics Profiling Data Sets: A Network Perspective

      • Akshay Bhat, Mohammed Dakna, Harald Mischak
      Pages 237-253

    4. The European Medicines Agency Experience with Biomarker Qualification

      • Efthymios Manolis, Armin Koch, Dieter Deforce, Spiros Vamvakas
      Pages 255-272

  5. Back Matter

    Pages 273-278
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Keywords

About this book

This second edition expands upon the  previous edition with current, detailed developments in the field and brings together a multi-disciplinary team of leading researchers to provide their latest protocols for clinical proteomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and Practical, Clinical Proteomics: Methods and Protocols, Second Edition presents  approaches that will serve as a reliable guide to researchers, including clinicians, chemists, molecular biologists, bioinformaticians and computational,  biologists,  and investigators working on biomarker development.

Editors and Affiliations

  • Biomedical Research Foundation, Academy of Athens, Athens, Greece

    Antonia Vlahou, Manousos Makridakis

Bibliographic Information

  • Book Title: Clinical Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Antonia Vlahou, Manousos Makridakis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1872-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1871-3Published: 11 November 2014

  • Softcover ISBN: 978-1-4939-4729-4Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1872-0Published: 10 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 278

  • Number of Illustrations: 12 b/w illustrations, 28 illustrations in colour

  • Topics: Protein Science, Proteomics

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