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  • © 2015

Chromosomal Mutagenesis

  • Includes all new cutting-edge methods and protocols for chromosomal mutagenesis research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1239)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xiv
  2. Genome Engineering Using Adeno-Associated Virus (AAV)

    • Rob Howes, Christine Schofield
    Pages 75-103
  3. Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform

    • Tomas Cermak, Colby G. Starker, Daniel F. Voytas
    Pages 133-159
  4. Ligation-Independent Cloning (LIC) Assembly of TALEN Genes

    • Jonathan L. Schmid-Burgk, Tobias Schmidt, Veit Hornung
    Pages 161-169
  5. Genome Engineering Using CRISPR-Cas9 System

    • Le Cong, Feng Zhang
    Pages 197-217
  6. Endogenous Gene Tagging with Fluorescent Proteins

    • John Fetter, Andrey Samsonov, Nathan Zenser, Fan Zhang, Hongyi Zhang, Dmitry Malkov
    Pages 231-240
  7. Gene Editing Using ssODNs with Engineered Endonucleases

    • Fuqiang Chen, Shondra M. Pruett-Miller, Gregory D. Davis
    Pages 251-265
  8. Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models

    • Victoria M. Bedell, Stephen C. Ekker
    Pages 291-305

About this book

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and fully updated, Chromosomal Mutagenesis, Second Edition aims to help speed scientific discovery and aid in the next advances in the field.

Editors and Affiliations

  • Department of Genetics, Genome Engineering and iPSC Center, Washington University School of Medicine, St. Louis, USA

    Shondra M. Pruett-Miller

Bibliographic Information

  • Book Title: Chromosomal Mutagenesis

  • Editors: Shondra M. Pruett-Miller

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1862-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1861-4Published: 19 November 2014

  • Softcover ISBN: 978-1-4939-5541-1Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1862-1Published: 19 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 333

  • Number of Illustrations: 22 illustrations in colour

  • Topics: Human Genetics, Genetic Engineering

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access