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Receptor Tyrosine Kinases

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Serena Germano

    1. Necker Hospital, Paris, France

    View editor publications

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  • Includes cutting-edge methods and protocols involving RTKs
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1233)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. RTKs Activation and Signalling

    1. Front Matter

      Pages 1-1
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    2. Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblotting

      • Martina McDermott, Norma O’Donovan
      Pages 3-14

    3. Analysis of Changes in Phosphorylation of Receptor Tyrosine Kinases: Antibody Arrays

      • Sweta Rani, Lorraine O’Driscoll
      Pages 15-23

    4. Analysis of Epidermal Growth Factor Receptor Dimerization by BS3 Cross-Linking

      • Harmony F. Turk, Robert S. Chapkin
      Pages 25-34

    5. Single-Molecule Optical Methods Analyzing Receptor Tyrosine Kinase Activation in Living Cells

      • Inhee Chung, Ira Mellman
      Pages 35-44

    6. Evaluation of the Dimerization Profiles of HER Tyrosine Kinases by Time-Resolved Förster Resonance Energy Transfer (TR-FRET)

      • Evelyne Lopez-Crapez, Alexandre Ho-Pun-Cheung, Patrick Garnero, Hervé Bazin
      Pages 45-55

    7. Applying the Proximity Ligation Assay (PLA) to Mouse Preimplantation Embryos for Identifying Protein-Protein Interactions In Situ

      • Ivan Bedzhov, Marc P. Stemmler
      Pages 57-64

  3. RTKs Aberrant Expression

    1. Front Matter

      Pages 65-65
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    2. Analysis of Receptor Tyrosine Kinase Gene Amplification on the Example of FGFR1

      • Diana Boehm, Anne von Mässenhausen, Sven Perner
      Pages 67-79

    3. Quantification of the Effects of Mutations on Receptor Tyrosine Kinase (RTK) Activation in Mammalian Cells

      • Lijuan He, Kalina Hristova
      Pages 81-87

  4. RTKs Trafficking and Negative Regulation

    1. Front Matter

      Pages 89-89
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    2. Cell Surface Biotinylation of Receptor Tyrosine Kinases to Investigate Intracellular Trafficking

      • Mathieu J. F. Crupi, Douglas S. Richardson, Lois M. Mulligan
      Pages 91-102

    3. Studying N-Linked Glycosylation of Receptor Tyrosine Kinases

      • Harri M. Itkonen, Ian G. Mills
      Pages 103-109

    4. Identification of Receptor Protein Tyrosine Phosphatases (RPTPs) as Regulators of Receptor Tyrosine Kinases (RTKs) Using an RPTP siRNA-RTK Substrate Screen

      • Hojin Lee, Anton M. Bennett
      Pages 111-120

    5. Downregulation of Receptor Tyrosine Kinases Through Ubiquitination: Analysis by Immunodetection

      • Noriaki Shimokawa, Noriyuki Koibuchi
      Pages 121-133

    6. Regulation of Receptor Tyrosine Kinases by miRNA: Overexpression of miRNA Using Lentiviral Inducible Expression Vectors

      • XiangDong Le, Andrew T. Huang, Yunyun Chen, Stephen Y. Lai
      Pages 135-147

  5. RTKs as Pharmacological Targets

    1. Front Matter

      Pages 149-149
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    2. Human Tumor Xenografts in Mouse as a Model for Evaluating Therapeutic Efficacy of Monoclonal Antibodies or Antibody-Drug Conjugate Targeting Receptor Tyrosine Kinases

      • Liang Feng, Wei Wang, Hang-Ping Yao, Jianwei Zhou, Ruiwen Zhang, Ming-Hai Wang
      Pages 151-159

    3. Receptor Tyrosine Kinase Targeting in Multicellular Spheroids

      • Susan Breslin, Lorraine O’Driscoll
      Pages 161-168
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Keywords

About this book

Receptor Tyrosine Kinases: Methods and Protocols explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies. Beginning with a section on activation and signaling of Receptor Tyrosine Kinases (RTKs), the book continues with chapters on aberrant expression of RTKs, trafficking and negative regulation, as well as RTKs as pharmacological targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Receptor Tyrosine Kinases: Methods and Protocols contains an up-to-date overview of the most relevant and widely used methods employed in this field, providing detailed protocols that molecular and cellular biologists could easily adopt in their research programs on RTKs.

Editors and Affiliations

  • Necker Hospital, Paris, France

    Serena Germano

Bibliographic Information

  • Book Title: Receptor Tyrosine Kinases

  • Book Subtitle: Methods and Protocols

  • Editors: Serena Germano

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1789-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1788-4Published: 16 October 2014

  • Softcover ISBN: 978-1-4939-5449-0Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1789-1Published: 15 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 181

  • Number of Illustrations: 7 b/w illustrations, 31 illustrations in colour

  • Topics: Protein Science, Membrane Biology

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