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Nuclear G-Protein Coupled Receptors

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Bruce G. Allen

    1. Montreal Heart Institute & Université de Montréal, Montreal, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Terence E. Hébert

    1. Pharmacology and Therapeutics, McGill University, Montreal, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1234)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Studies of Intracellular Angiotensin II

    • Richard N. Re, Julia L. Cook
    Pages 1-8

  3. Single-Cell Microinjection Coupled to Confocal Microscopy to Characterize Nuclear Membrane Receptors in Freshly Isolated Cardiomyocytes

    • Clémence Merlen, Jonathan Ledoux
    Pages 9-16

  4. Design and Application of Light-Activated Probes for Cellular Signaling

    • David Chatenet, Steve Bourgault, Alain Fournier
    Pages 17-30

  5. Using Caged Ligands to Study Intracrine Endothelin Signaling in Intact Cardiac Myocytes

    • Clémence Merlen, Louis R. Villeneuve, Bruce G. Allen
    Pages 31-41

  6. Quantification of Catecholamine Uptake in Adult Cardiac Myocytes

    • Erika F. Dahl, Casey D. Wright, Timothy D. O’Connell
    Pages 43-52

  7. Characterization of the Interaction Between the Prostaglandin D2 DP1 Receptor and the Intracellular l-Prostaglandin D Synthase

    • Chantal Binda, Jean-Luc Parent
    Pages 53-67

  8. Isolation and Study of Cardiac Nuclei from Canine Myocardium and Adult Ventricular Myocytes

    • Artavazd Tadevosyan, Bruce G. Allen, Stanley Nattel
    Pages 69-80

  9. High Resolution Imaging and Function of Nuclear G Protein-Coupled Receptors (GPCRs)

    • Vikrant K. Bhosle, Fernand Gobeil Jr., Jose Carlos Rivera, Alfredo Ribeiro-da-Silva, Sylvain Chemtob
    Pages 81-97

  10. Biochemical Fractionation of Membrane Receptors in the Nucleus

    • Ying-Nai Wang, Longfei Huo, Jennifer L. Hsu, Mien-Chie Hung
    Pages 99-112

  11. Functional G Protein-Coupled Receptors on Nuclei from Brain and Primary Cultured Neurons

    • Yuh-Jiin I. Jong, Karen L. O’Malley
    Pages 113-121

  12. Automated Microscopy of Cardiac Myocyte Hypertrophy: A Case Study on the Role of Intracellular α-Adrenergic Receptors

    • Karen A. Ryall, Jeffrey J. Saucerman
    Pages 123-134

  13. Measuring Intranuclear and Nuclear Envelope [Ca2+] vs. Cytosolic [Ca2+]

    • Senka Ljubojević, Donald M. Bers
    Pages 135-147

  14. Assessing GPCR and G Protein Signaling to the Nucleus in Live Cells Using Fluorescent Biosensors

    • Julie Bossuyt, Donald M. Bers
    Pages 149-159

  15. Tandem Affinity Purification to Identify Cytosolic and Nuclear Gβγ-Interacting Proteins

    • Rhiannon Campden, Darlaine Pétrin, Mélanie Robitaille, Nicolas Audet, Sarah Gora, Stéphane Angers et al.
    Pages 161-184

  16. Examining the Effects of Nuclear GPCRs on Gene Expression Using Isolated Nuclei

    • George Vaniotis, Sarah Gora, André Nantel, Terence E. Hébert, Bruce G. Allen
    Pages 185-195

  17. Trafficking and Function of GPCRs in the Endosomal Compartment

    • Davide Calebiro, Amod Godbole, Sandra Lyga, Martin J. Lohse
    Pages 197-211

  18. Back Matter

    Pages 213-215
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Keywords

About this book

Nuclear G-Protein Coupled Receptors: Methods and Protocols is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (Ang II), covered in depth in various chapters. Methodology to study the subcellular localization and function of GPCRs and other signaling systems is provided, as well as numerous chapters focusing on methods designed to understand signaling mediated by nuclear and other internal GPCRs. Methods are also described to study the formation of second messengers such as cAMP and to study the trafficking of receptors from the cell surface. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Nuclear G-Protein Coupled Receptors: Methods and Protocols seeks to serve both professionals and novices with state-of-the-art approaches to characterize what is becoming a common theme in cellular signaling.

Editors and Affiliations

  • Montreal Heart Institute & Université de Montréal, Montreal, Canada

    Bruce G. Allen

  • Pharmacology and Therapeutics, McGill University, Montreal, Canada

    Terence E. Hébert

Bibliographic Information

  • Book Title: Nuclear G-Protein Coupled Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Bruce G. Allen, Terence E. Hébert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1755-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1754-9Published: 11 October 2014

  • Softcover ISBN: 978-1-4939-5546-6Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1755-6Published: 10 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 215

  • Number of Illustrations: 28 b/w illustrations, 15 illustrations in colour

  • Topics: Protein Science

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