Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1232)
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Table of contents (21 protocols)
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Front Matter
About this book
This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico. Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.
Keywords
- Cell membranes
- X-Ray scattering
- archetypes
- artificial membranes
- asymmetric planar lipid bilayers
- atomic force microscopy
- cellular membranes
- component lipids
- fluorescence correlation spectroscopy
- fluorescent probes
- lipid phase behaviour
- nuclear magnetic resonance (NMR)
- photobleaching
- tethered lipid bilayers
Editors and Affiliations
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Department of Physics and Randall Division of Cell and Molecular Biophysics, King’s College London, London, United Kingdom
Dylan M. Owen
Bibliographic Information
Book Title: Methods in Membrane Lipids
Editors: Dylan M. Owen
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1752-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-1751-8Published: 21 October 2014
Softcover ISBN: 978-1-4939-5399-8Published: 22 September 2016
eBook ISBN: 978-1-4939-1752-5Published: 20 October 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 327
Number of Illustrations: 28 b/w illustrations, 15 illustrations in colour
Topics: Cell Biology, Membrane Biology, Lipidology