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Plant Virology Protocols

New Approaches to Detect Viruses and Host Responses

  • Book
  • © 2015

Overview

Editors:
  1. Ichiro Uyeda

    1. Hokkaido University, Sapporo, Japan

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    You can also search for this editor in PubMed   Google Scholar

  2. Chikara Masuta

    1. Hokkaido University, Sapporo, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes new cutting-edge methods and protocols in plant virology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1236)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Detection of Plant Viruses in Mixed Infection by a Macroarray-Assisted Method

    • Hanako Shimura, Kazuyoshi Furuta, Chikara Masuta
    Pages 1-11

  3. RT-PCR and Real-Time RT-PCR Methods for the Detection of Potato Virus Y in Potato Leaves and Tubers

    • Tyler D. B. MacKenzie, Xianzhou Nie, Mathuresh Singh
    Pages 13-26

  4. A New Method to Isolate Total dsRNA

    • Go Atsumi, Ken-Taro Sekine, Kappei Kobayashi
    Pages 27-37

  5. Multiplex RT-PCR Method for the Simultaneous Detection of Nine Grapevine Viruses

    • Giorgio Gambino
    Pages 39-47

  6. Detection Methods for Rice Viruses by a Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)

    • Takahide Sasaya
    Pages 49-59

  7. Real-Time PCR Protocols for the Quantification of the Begomovirus Tomato Yellow Leaf Curl Sardinia Virus in Tomato Plants and in Its Insect Vector

    • Emanuela Noris, Laura Miozzi
    Pages 61-72

  8. Detection and Analysis of Non-retroviral RNA Virus-Like Elements in Plant, Fungal, and Insect Genomes

    • Hideki Kondo, Sotaro Chiba, Nobuhiro Suzuki
    Pages 73-88

  9. Detection of Plant Viruses in Natural Environments by Using RNA-Seq

    • Atsushi J. Nagano, Mie N. Honjo, Motohiro Mihara, Masanao Sato, Hiroshi Kudoh
    Pages 89-98

  10. Cloning and Profiling of Small RNAs from Cucumber Mosaic Virus Satellite RNA

    • Yuan-Yuan Fang, Neil A. Smith, Jian-Hua Zhao, Joanne R. M. Lee, Hui-Shan Guo, Ming-Bo Wang
    Pages 99-109

  11. Drawing siRNAs of Viral Origin Out from Plant siRNAs Libraries

    • Laura Miozzi, Vitantonio Pantaleo
    Pages 111-123

  12. Viral Detection by High-Throughput Sequencing

    • Daisuke Motooka, Shota Nakamura, Katsuro Hagiwara, Takaaki Nakaya
    Pages 125-134

  13. Analysis and Application of Viroid-Specific Small RNAs Generated by Viroid-Inducing RNA Silencing

    • Charith Raj Adkar-Purushothama, Zhixiang Zhang, Shifang Li, Teruo Sano
    Pages 135-170

  14. Detection and Characterization of Mycoviruses in Arbuscular Mycorrhizal Fungi by Deep-Sequencing

    • Tatsuhiro Ezawa, Yoji Ikeda, Hanako Shimura, Chikara Masuta
    Pages 171-180

  15. SuperSAGE as an Analytical Tool for Host and Viral Gene Expression

    • Hideo Matsumura, Detlev H. Krüger, Günter Kahl, Ryohei Terauchi
    Pages 181-195

  16. Microarray Analysis of R-Gene-Mediated Resistance to Viruses

    • Takeaki Ishihara, Yukiyo Sato, Hideki Takahashi
    Pages 197-218

  17. Construction of Infectious cDNA Clones Derived from the Potyviruses Clover Yellow Vein Virus and Bean Yellow Mosaic Virus

    • Kenji S. Nakahara, Kei Nishino, Ichiro Uyeda
    Pages 219-227

  18. Virus-Induced Gene Silencing of N Gene in Tobacco by Apple Latent Spherical Virus Vectors

    • Chunjiang Li, Nobuyuki Yoshikawa
    Pages 229-240

  19. Simplified Methods for the Construction of RNA and DNA Virus Infectious Clones

    • Tatsuya Nagata, Alice Kazuko Inoue-Nagata
    Pages 241-254

  20. Efficient Double-Stranded RNA Production Methods for Utilization in Plant Virus Control

    • Andreas E. Voloudakis, Maria C. Holeva, L. Peter Sarin, Dennis H. Bamford, Marisol Vargas, Minna M. Poranen et al.
    Pages 255-274
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Keywords

About this book

Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses will be an invaluable guide to researchers working in the field of plant sciences.

Editors and Affiliations

  • Hokkaido University, Sapporo, Japan

    Ichiro Uyeda, Chikara Masuta

Bibliographic Information

  • Book Title: Plant Virology Protocols

  • Book Subtitle: New Approaches to Detect Viruses and Host Responses

  • Editors: Ichiro Uyeda, Chikara Masuta

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1743-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1742-6Published: 07 October 2014

  • Softcover ISBN: 978-1-4939-5540-4Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1743-3Published: 06 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 292

  • Number of Illustrations: 38 b/w illustrations, 32 illustrations in colour

  • Topics: Plant Sciences, Virology

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