Editors:
- Emphasizes agricultural crops or plant species with economic values
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1223)
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Table of contents (29 protocols)
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Front Matter
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Cereal Plants
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Front Matter
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Legume Plants
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Front Matter
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About this book
Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.
Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.
Editors and Affiliations
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Plant Sciences Institute, and Department of Agronomy, Iowa State University, Center for Plant Transformation, Ames, USA
Kan Wang
Bibliographic Information
Book Title: Agrobacterium Protocols
Book Subtitle: Volume 1
Editors: Kan Wang
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1695-5
Publisher: Springer New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-1694-8Published: 09 October 2014
Softcover ISBN: 978-1-4939-4541-2Published: 23 August 2016
eBook ISBN: 978-1-4939-1695-5Published: 09 October 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XIV, 368
Number of Illustrations: 13 b/w illustrations, 29 illustrations in colour
Topics: Plant Sciences, Plant Genetics and Genomics, Genetic Engineering