Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1224)
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Table of contents (29 protocols)
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Front Matter
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Industrial Plants
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Front Matter
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About this book
Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.
Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers in both fundamental and applied biology.
Editors and Affiliations
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Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, USA
Kan Wang
Bibliographic Information
Book Title: Agrobacterium Protocols
Book Subtitle: Volume 2
Editors: Kan Wang
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1658-0
Publisher: Springer New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-1657-3Published: 22 November 2014
Softcover ISBN: 978-1-4939-4544-3Published: 23 August 2016
eBook ISBN: 978-1-4939-1658-0Published: 21 November 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XVI, 378
Number of Illustrations: 6 b/w illustrations, 41 illustrations in colour
Topics: Plant Sciences, Plant Genetics and Genomics, Genetic Engineering