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Bacterial Artificial Chromosomes

  • Book
  • © 2015

Overview

Editors:
  1. Kumaran Narayanan

    1. Monash University Malaysia, Bandar Sunway, Malaysia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1227)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. BAC Construction and Characterization

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. From Selective Full-Length Genes Isolation by TAR Cloning in Yeast to Their Expression from HAC Vectors in Human Cells

      • Natalay Kouprina, Nicholas C. O. Lee, Artem V. Kononenko, Alexander Samoshkin, Vladimir Larionov
      Pages 3-26

    3. Recombineering Linear BACs

      • Qingwen Chen, Kumaran Narayanan
      Pages 27-54

    4. BAC Sequencing Using Pooled Methods

      • Christopher A. Saski, F. Alex Feltus, Laxmi Parida, Niina Haiminen
      Pages 55-67

  3. Modification of BACs

    1. Front Matter

      Pages 69-69
      Download chapter PDF

    2. Making BAC Transgene Constructs with Lambda-Red Recombineering System for Transgenic Animals or Cell Lines

      • Scott Holmes, Suzanne Lyman, Jen-Kang Hsu, JrGang Cheng
      Pages 71-98

    3. Directing Enhancer-Traps and iTol2 End-Sequences to Deleted BAC Ends with loxP- and lox511-Tn10 Transposons

      • Pradeep K. Chatterjee
      Pages 99-122

    4. Recombining Overlapping BACs into Single Large BACs

      • George Kotzamanis, Athanassios Kotsinas
      Pages 123-139

  5. Applications of BACs in Model Organisms, Medical Genetics, and Drug Discovery

    1. Front Matter

      Pages 231-231
      Download chapter PDF

    2. A Recombineering-Based Gene Tagging System for Arabidopsis

      • Jose M. Alonso, Anna N. Stepanova
      Pages 233-243

    3. BAC Transgenic Zebrafish for Transcriptional Promoter and Enhancer Studies

      • Petra Kraus, Cecilia L. Winata, Thomas Lufkin
      Pages 245-258

    4. BACs-on-Beads™ (BoBs™) Assay for the Genetic Evaluation of Prenatal Samples and Products of Conception

      • Francesca Romana Grati, François Vialard, Susan Gross
      Pages 259-278

    5. New BAC Probe Set to Narrow Down Chromosomal Breakpoints in Small and Large Derivative Chromosomes, Especially Suited for Mosaic Conditions

      • Ahmed B. Hamid, Xiaobo Fan, Nadezda Kosyakova, Gopakumar Radhakrishnan, Thomas Liehr, Tatyana Karamysheva
      Pages 279-287

    6. BAC-Probes Applied for Characterization of Fragile Sites (FS)

      • Kristin Mrasek, Kathleen Wilhelm, Luciana G. Quintana, Luise Theuss, Thomas Liehr, Andreja Leskovac et al.
      Pages 289-298
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Keywords

About this book

Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs. Updated chapters included in this edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model organisms, and medical genetics and drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and tips to troubleshoot and avoid known pitfalls.

Authoritative and cutting-edge, Bacterial Artificial Chromosomes, Second Edition seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies.

Editors and Affiliations

  • Monash University Malaysia, Bandar Sunway, Malaysia

    Kumaran Narayanan

Bibliographic Information

  • Book Title: Bacterial Artificial Chromosomes

  • Editors: Kumaran Narayanan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1652-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1651-1Published: 20 September 2014

  • Softcover ISBN: 978-1-4939-5430-8Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1652-8Published: 19 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 348

  • Number of Illustrations: 12 b/w illustrations, 36 illustrations in colour

  • Topics: Cell Biology, Gene Function

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