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Osteoporosis and Osteoarthritis

  • Book
  • © 2015

Overview

Editors:
  1. Jennifer J. Westendorf

    1. Mayo Clinic, Rochester, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Andre J. van Wijnen

    1. Mayo Clinic, Rochester, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1226)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Cell Biology

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Isolation of Osteocytes from Mature and Aged Murine Bone

      • Amber Rath Stern, Lynda F. Bonewald
      Pages 3-10

    3. Primary Murine Growth Plate and Articular Chondrocyte Isolation and Cell Culture

      • Jennifer H. Jonason, Donna Hoak, Regis J. O’Keefe
      Pages 11-18

    4. Isolating Endosteal Mesenchymal Progenitors from Rodent Long Bones

      • Ji Zhu, Valerie A. Siclari, Ling Qin
      Pages 19-29

    5. Engineering Cartilage Tissue by Pellet Coculture of Chondrocytes and Mesenchymal Stromal Cells

      • Ling Wu, Janine N. Post, Marcel Karperien
      Pages 31-41

    6. Generation of Induced Pluripotent Stem Cells

      • David R. Deyle
      Pages 43-58

  3. Molecular Biology

    1. Front Matter

      Pages 59-59
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    2. Profiling Histone Modifications by Chromatin Immunoprecipitation Coupled to Deep Sequencing in Skeletal Cells

      • Mark B. Meyer, Nancy A. Benkusky, J. Wesley Pike
      Pages 61-70

    3. Identification of microRNAs in Human Plasma

      • Bram C. van der Eerden, Rodrigo D. Alves, Christel E. Kockx, Zeliha Ozgur, Marijke Schreuders-Koedam, Jeroen van de Peppel et al.
      Pages 71-85

    4. Immunohistochemistry of Skeletal Tissues

      • Crystal Idleburg, Elizabeth N. DeLassus, Deborah V. Novack
      Pages 87-95

  4. Biomechanics and Imaging

    1. Front Matter

      Pages 97-97
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    2. In Vivo Axial Loading of the Mouse Tibia

      • Katherine M. Melville, Alexander G. Robling, Marjolein C. H. van der Meulen
      Pages 99-115

    3. Four-Point Bending Protocols to Study the Effects of Dynamic Strain in Osteoblastic Cells In Vitro

      • Gabriel L. Galea, Joanna S. Price
      Pages 117-130

    4. EPIC-μCT Imaging of Articular Cartilage

      • Angela S. P. Lin, Giuliana E. Salazar-Noratto, Robert E. Guldberg
      Pages 131-140

  5. In Vivo Models of Disease and Repair

    1. Front Matter

      Pages 141-141
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    2. Mouse Models of Osteoarthritis: Surgical Model of Posttraumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus

      • Kirsty L. Culley, Cecilia L. Dragomir, Jun Chang, Elisabeth B. Wondimu, Jonathan Coico, Darren A. Plumb et al.
      Pages 143-173

    3. Assessment of Knee Joint Pain in Experimental Rodent Models of Osteoarthritis

      • Margaret J. Piel, Jeffrey S. Kroin, Hee-Jeong Im
      Pages 175-181

    4. Induction of Fully Stabilized Cortical Bone Defects to Study Intramembranous Bone Regeneration

      • Meghan E. McGee-Lawrence, David F. Razidlo
      Pages 183-192

    5. Surgical Procedures and Experimental Outcomes of Closed Fractures in Rodent Models

      • Hicham Drissi, David N. Paglia
      Pages 193-211
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Keywords

About this book

In Osteoporosis and Osteoarthritis expert investigators detail modern tools to enhance discovery in bone and cartilage biology and promote translational research in osteoporosis and osteoarthritis. New chapters describe methods to generate induced pluripotent stem cells, perform high-throughput chromatin analysis, assess loads on bone cells in vivo and in vitro, use state-of-the art imaging techniques to measure articular cartilage thickness and biochemical properties, and establish animal models of osteoarthritis and bone healing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical,  Osteoporosis and Osteoarthritis shares successful techniques that can be applied to the study of either disease.

Editors and Affiliations

  • Mayo Clinic, Rochester, USA

    Jennifer J. Westendorf, Andre J. van Wijnen

Bibliographic Information

  • Book Title: Osteoporosis and Osteoarthritis

  • Editors: Jennifer J. Westendorf, Andre J. van Wijnen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1619-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media. New York 2015

  • Hardcover ISBN: 978-1-4939-1618-4Published: 21 October 2014

  • Softcover ISBN: 978-1-4939-5527-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1619-1Published: 20 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 214

  • Number of Illustrations: 8 b/w illustrations, 38 illustrations in colour

  • Topics: Molecular Medicine, Orthopedics

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