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Nuclear Reprogramming

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Nathalie Beaujean

    1. INRA, Jouy-en-Josas, France

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  2. Hélène Jammes

    1. INRA, Jouy-en-Josas, France

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    You can also search for this editor in PubMed   Google Scholar

  3. Alice Jouneau

    1. INRA, Jouy-en-Josas, France

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    You can also search for this editor in PubMed   Google Scholar

  • Includes fully updated, cutting-edge methods and protocols for nuclear reprogramming
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1222)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Nuclear Transfer in the Mouse

    • Vincent Brochard, Zichuan Liu
    Pages 1-14

  3. Nuclear Transfer in Rabbit

    • Nathalie Daniel, Patrick Chesné
    Pages 15-23

  4. Nuclear Transfer in Ruminants

    • Joon-Hee Lee, Walid E. Maalouf
    Pages 25-36

  5. Nuclear Transfer and Transgenesis in the Pig

    • Mayuko Kurome, Barbara Kessler, Annegret Wuensch, Hiroshi Nagashima, Eckhard Wolf
    Pages 37-59

  6. Embryonic Stem Cell–Somatic Cell Fusion and Postfusion Enucleation

    • Huseyin Sumer, Paul J. Verma
    Pages 61-69

  7. Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte

    • Jerome Jullien
    Pages 71-82

  8. Assessing the Quality of Donor Cells: Karyotyping Methods

    • Amélie Bonnet-Garnier, Anne-Clémence Veillard, Bertrand Bed’Hom, Hélène Hayes, Janice Britton-Davidian
    Pages 83-99

  9. Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer

    • Eiji Mizutani, Sayaka Wakayama, Teruhiko Wakayama
    Pages 101-111

  10. Fluorescent Immunodetection of Epigenetic Modifications on Preimplantation Mouse Embryos

    • Claire Boulesteix, Nathalie Beaujean
    Pages 113-126

  11. Visualization of Epigenetic Modifications in Preimplantation Embryos

    • Hiroshi Kimura, Kazuo Yamagata
    Pages 127-147

  12. Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer

    • Sebastian T. Balbach, Michele Boiani
    Pages 149-159

  13. Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming

    • Olga Østrup, Hanne S. Pedersen, Hanne M. Holm, Poul Hyttel
    Pages 161-174

  14. Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining

    • Sabine Chauveau, Claire Chazaud
    Pages 175-180

  15. Gene Expression Analysis in Early Embryos Through Reverse Transcription Quantitative PCR (RT-qPCR)

    • Nathalie Peynot, Véronique Duranthon, Daulat Raheem Khan
    Pages 181-196

  16. Studying Bovine Early Embryo Transcriptome by Microarray

    • Isabelle Dufort, Claude Robert, Marc-André Sirard
    Pages 197-208

  17. Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level

    • Annick Lefèvre, Thierry Blachère
    Pages 209-226

  18. Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos

    • John Arne Dahl, Arne Klungland
    Pages 227-245

  19. Assessing Reprogramming by Chimera Formation and Tetraploid Complementation

    • Xin Li, Bao-long Xia, Wei Li, Qi Zhou
    Pages 247-254

  20. Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology

    • Bernd Püschel, Alice Jouneau
    Pages 255-265
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Keywords

About this book

Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Nuclear Reprogramming: Methods and Protocols, Second Edition will be of interest not only to cloners but also to researchers concerned with studying the development of embryos.

Editors and Affiliations

  • INRA, Jouy-en-Josas, France

    Nathalie Beaujean, Hélène Jammes, Alice Jouneau

Bibliographic Information

  • Book Title: Nuclear Reprogramming

  • Book Subtitle: Methods and Protocols

  • Editors: Nathalie Beaujean, Hélène Jammes, Alice Jouneau

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1594-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1593-4Published: 07 October 2014

  • Softcover ISBN: 978-1-4939-5441-4Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1594-1Published: 06 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 285

  • Number of Illustrations: 25 b/w illustrations, 43 illustrations in colour

  • Topics: Cell Biology, Genetic Engineering

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