Overview
- Includes cutting-edge methods and protocols involving plasmodesmata research in plant science
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1217)
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Table of contents (21 protocols)
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Front Matter
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Imaging of Plasmodesmata
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Front Matter
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Structural Analysis of Plasmodesmata
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Front Matter
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Analysis of Plasmodesmata Conductivity and Regulation
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Front Matter
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Studying Macromolecular Trafficking
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Front Matter
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Keywords
About this book
Plasmodesmata: Methods and Protocols provides a unique compendium of currently applied methods to explore plasmodesmata (PD). Opening with overviews of PD structure and their function during development as well as the role of viruses as models for addressing cellular mechanisms in macromolecular trafficking and movement through PD, the book continues with detailed experimental protocols for imaging PD, the isolation and structural analysis of PD, the analysis of PD conductivity and regulation, and for studying cellular mechanisms by which macromolecules are targeted to PD and transported through the channel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and easy to use, Plasmodesmata: Methods and Protocols delivers detailed information about specific practical procedures in order to enable their reproduction and application in PD-related research in laboratories around the world.
Editors and Affiliations
Bibliographic Information
Book Title: Plasmodesmata
Book Subtitle: Methods and Protocols
Editors: Manfred Heinlein
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1523-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-1522-4Published: 07 October 2014
Softcover ISBN: 978-1-4939-4643-3Published: 30 April 2017
eBook ISBN: 978-1-4939-1523-1Published: 06 October 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 346
Number of Illustrations: 14 b/w illustrations, 39 illustrations in colour
Topics: Plant Sciences