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In Situ Hybridization Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Boye Schnack Nielsen

    1. Molecular Histology, Bioneer A/S, Horsholm, Denmark

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1211)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Fixation/Permeabilization Procedure for mRNA In Situ Hybridization of Zebrafish Whole-Mount Oocytes, Embryos, and Larvae

    • Ricardo Fuentes, Juan Fernández
    Pages 1-13

  3. MicroRNA In Situ Hybridization on Whole-Mount Preimplantation Embryos

    • Karen Goossens, Luc Peelman, Ann Van Soom
    Pages 15-25

  4. Whole-Mount In Situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies

    • Eleni Dakou, Nele Vanbekbergen, Sara Corradi, Caroline R. Kemp, Erik Willems, Luc Leyns
    Pages 27-40

  5. Whole-Mount In Situ Hybridization Using DIG-Labeled Probes in Planarian

    • Agnieszka Rybak-Wolf, Jordi Solana
    Pages 41-51

  6. In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae

    • Bernard Thisse, Christine Thisse
    Pages 53-67

  7. LNA-Based In Situ Hybridization Detection of mRNAs in Embryos

    • Diana K. Darnell, Parker B. Antin
    Pages 69-76

  8. Chromogen Detection of microRNA in Frozen Clinical Tissue Samples Using LNA™ Probe Technology

    • Boye Schnack Nielsen, Trine Møller, Kim Holmstrøm
    Pages 77-84

  9. MicroRNA In Situ Hybridization in Tissue Microarrays

    • Julia J. Turnock-Jones, John P. C. Le Quesne
    Pages 85-93

  10. Fluorescence In Situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections

    • Asli Silahtaroglu
    Pages 95-102

  11. Sensitive and Specific In Situ Hybridization for Early Drug Discovery

    • Pernille A. Usher, Elisabeth D. Galsgaard, Kimberly Kruse, Jishu Wang, Berit O. Krogh, Jette Mandelbaum et al.
    Pages 103-123

  12. Zinc-Based Fixation for High-Sensitivity In Situ Hybridization: A Nonradioactive Colorimetric Method for the Detection of Rare Transcripts on Tissue Sections

    • Electra Stylianopoulou, George Skavdis, Maria Grigoriou
    Pages 125-138

  13. Dual-Color Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope

    • Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielsen et al.
    Pages 139-149

  14. Fully Automated Fluorescence-Based Four-Color Multiplex Assay for Co-detection of MicroRNA and Protein Biomarkers in Clinical Tissue Specimens

    • Lorenzo F. Sempere
    Pages 151-170

  15. Multiplexed miRNA Fluorescence In Situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues

    • Neil Renwick, Pavol Cekan, Claudia Bognanni, Thomas Tuschl
    Pages 171-187

  16. Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris® RNA Fluorescence In Situ Hybridization in Adherent Cells

    • Sally R. Coassin, Arturo V. Orjalo Jr., Sheila J. Semaan, Hans E. Johansson
    Pages 189-199

  17. Quantitative Ultrasensitive Bright-Field RNA In Situ Hybridization with RNAscope

    • Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielsen et al.
    Pages 201-212

  18. Identification of Low-Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia

    • Sergei Zolotukhin
    Pages 213-228

  19. In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes

    • Kathleen T. Montone
    Pages 229-235

  20. In Situ Hybridization Freeze-Assisted Punches (IFAP): Technique for Liquid-Based Tissue Extraction from Thin Slide-Mounted Sections for DNA Methylation Analysis

    • Lawrence S. Own, Paresh D. Patel
    Pages 237-243
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Keywords

About this book

In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.

Editors and Affiliations

  • Molecular Histology, Bioneer A/S, Horsholm, Denmark

    Boye Schnack Nielsen

Bibliographic Information

  • Book Title: In Situ Hybridization Protocols

  • Editors: Boye Schnack Nielsen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1459-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1458-6Published: 13 September 2014

  • Softcover ISBN: 978-1-4939-5526-8Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1459-3Published: 13 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XI, 275

  • Number of Illustrations: 4 b/w illustrations, 52 illustrations in colour

  • Topics: Human Genetics

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