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  • © 2015

Regulatory Non-Coding RNAs

Methods and Protocols

  • Includes cutting-edge methods and protocols on non-coding RNAs
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1206)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
  2. Diverse Functions and Mechanisms of Mammalian Long Noncoding RNAs

    • Callie R. Merry, Courtney Niland, Ahmad M. Khalil
    Pages 1-14
  3. Sequence-Defined Oligoaminoamides for the Delivery of siRNAs

    • Dian-Jang Lee, Ernst Wagner, Taavi Lehto
    Pages 15-27
  4. A Biochemical Approach to Identify Direct MicroRNA Targets

    • Murugan Subramanian, Xiao Ling Li, Toshifumi Hara, Ashish Lal
    Pages 29-37
  5. RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells

    • Vidisha Tripathi, Jingyi Fei, Taekjip Ha, Kannanganattu V. Prasanth
    Pages 123-136
  6. Locating RNAs In Situ with FISH-STIC Probes

    • John R. Sinnamon, Kevin Czaplinski
    Pages 137-148
  7. Back Matter

    Pages 179-181

About this book

Regulatory Non-Coding RNAs: Methods and Protocols offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression. After a review of the field, this detailed volume continues with methods useful for the study of siRNAs, microRNAs and their targets, techniques concerned with long non-coding RNAs, as well as studies of the critical parameters of functional non-coding RNA protein-RNA interactions and the environment in which they act. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips for troubleshooting and avoiding known pitfalls.

Dependable and easy to use, Regulatory Non-Coding RNAs: Methods and Protocols provides a current, state-of-the-art collection of methods and approaches that will be of value to researchers in this expanding and fascinating field.

Editors and Affiliations

  • University of Connecticut Health Center, Farmington, USA

    Gordon G. Carmichael

Bibliographic Information

  • Book Title: Regulatory Non-Coding RNAs

  • Book Subtitle: Methods and Protocols

  • Editors: Gordon G. Carmichael

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1369-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1368-8Published: 20 September 2014

  • Softcover ISBN: 978-1-4939-4184-1Published: 22 September 2016

  • eBook ISBN: 978-1-4939-1369-5Published: 20 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 181

  • Number of Illustrations: 29 b/w illustrations, 10 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access