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Yeast Genetics

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Jeffrey S. Smith

    1. Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Daniel J. Burke

    1. Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1205)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

    • R. Daniel Gietz
    Pages 1-12

  3. Tetrad, Random Spore, and Molecular Analysis of Meiotic Segregation and Recombination

    • Michael Lichten
    Pages 13-28

  4. PCR Mutagenesis and Gap Repair in Yeast

    • Mark Weir, Jill B. Keeney
    Pages 29-35

  5. PCR-Mediated Epitope Tagging of Genes in Yeast

    • Radhika Mathur, Peter Kaiser
    Pages 37-44

  6. Manipulating the Yeast Genome: Deletion, Mutation, and Tagging by PCR

    • Jennifer M. Gardner, Sue L. Jaspersen
    Pages 45-78

  7. Preparation of Yeast Cells for Live-Cell Imaging and Indirect Immunofluorescence

    • Lucy F. Pemberton
    Pages 79-90

  8. Single Yeast Cell Imaging

    • Heimo Wolinski, Sepp D. Kohlwein
    Pages 91-109

  9. Microfluidic Platforms for Generating Dynamic Environmental Perturbations to Study the Responses of Single Yeast Cells

    • Anjali Bisaria, Pascal Hersen, Megan N. McClean
    Pages 111-129

  10. Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations

    • Brian Haarer, David C. Amberg
    Pages 131-142

  11. Synthetic Genetic Array Analysis for Global Mapping of Genetic Networks in Yeast

    • Elena Kuzmin, Sara Sharifpoor, Anastasia Baryshnikova, Michael Costanzo, Chad L. Myers, Brenda J. Andrews et al.
    Pages 143-168

  12. Chemical Genetic and Chemogenomic Analysis in Yeast

    • Namal V. C. Coorey, Liam D. P. Sampson, Jacqueline M. Barber, David S. Bellows
    Pages 169-186

  13. Phenomic Assessment of Genetic Buffering by Kinetic Analysis of Cell Arrays

    • John Rodgers, Jingyu Guo, John L. Hartman IV
    Pages 187-208

  14. Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast

    • Badri Nath Singh, Michael Hampsey
    Pages 209-218

  15. Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast

    • Maria D. Mayán, Luis Aragón
    Pages 219-229

  16. Global Analysis of Transcription Factor-Binding Sites in Yeast Using ChIP-Seq

    • Philippe Lefrançois, Jennifer E. G. Gallagher, Michael Snyder
    Pages 231-255

  17. High-Density Tiling Microarray Analysis of the Full Transcriptional Activity of Yeast

    • Lior David, Sandra Clauder-Münster, Lars M. Steinmetz
    Pages 257-273

  18. Analysis of Silencing in Saccharomyces cerevisiae

    • Andrew Miller, Ann L. Kirchmaier
    Pages 275-302

  19. A User’s Guide to the Ribosomal DNA in Saccharomyces cerevisiae

    • Joseph M. Johnson, Jeffrey S. Smith, David A. Schneider
    Pages 303-328

  20. Two-Dimensional Agarose Gel Electrophoresis for Analysis of DNA Replication

    • Sandra K. Villwock, Oscar M. Aparicio
    Pages 329-340
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Keywords

About this book

Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.

Editors and Affiliations

  • Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, USA

    Jeffrey S. Smith, Daniel J. Burke

Bibliographic Information

  • Book Title: Yeast Genetics

  • Book Subtitle: Methods and Protocols

  • Editors: Jeffrey S. Smith, Daniel J. Burke

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1363-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1362-6Published: 12 September 2014

  • Softcover ISBN: 978-1-4939-5182-6Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1363-3Published: 11 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 378

  • Number of Illustrations: 42 b/w illustrations, 15 illustrations in colour

  • Topics: Microbiology

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