Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1204)
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Table of contents (21 protocols)
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Front Matter
Keywords
About this book
Steroid Receptors: Methods and Protocols presents a selection of techniques that have been recently applied to the analysis of steroid receptors, powerful tools for the advancement of our understanding of both the mechanisms regulating gene transcription and the rapid signaling responses of tissues to signals. Research in this area has generated a wealth of data allowing the elucidation of steroid receptor mechanisms and improving the treatment of many endocrine disorders, above all cancers. Chapters cover methods to analyze gene transcription, chromatin and proteomic modifications, extra-nuclear signaling regulation, development of cell and animal models, and preparation of new antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Steroid Receptors: Methods and Protocols offers an updated view on a variety of modern methods that will hopefully contribute to improving our knowledge on the integration of steroid receptors in single or different functionally connected cellular contexts under a variety of physiological and pathological conditions, above all tumors.
Editors and Affiliations
Bibliographic Information
Book Title: Steroid Receptors
Book Subtitle: Methods and Protocols
Editors: Gabriella Castoria, Ferdinando Auricchio
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1346-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1345-9Published: 02 September 2014
Softcover ISBN: 978-1-4939-4845-1Published: 17 September 2016
eBook ISBN: 978-1-4939-1346-6Published: 02 September 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 248
Number of Illustrations: 15 b/w illustrations, 35 illustrations in colour
Topics: Biochemistry, general