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  • © 2014

Biomimetics and Stem Cells

Methods and Protocols

  • Includes cutting-edge methods and protocols on the use of biomimetic systems with stem cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1202)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
  2. Derivation and Network Formation of Vascular Cells from Human Pluripotent Stem Cells

    • Sravanti Kusuma, Bria Macklin, Sharon Gerecht
    Pages 1-9
  3. High-Throughput Cell Aggregate Culture for Stem Cell Chondrogenesis

    • Ahmad E. Abu-Hakmeh, Leo Q. Wan
    Pages 11-19
  4. Microfluidic Device to Culture 3D In Vitro Human Capillary Networks

    • Monica L. Moya, Luis F. Alonzo, Steven C. George
    Pages 21-27
  5. Multifunction Co-culture Model for Evaluating Cell–Cell Interactions

    • Danielle R. Bogdanowicz, Helen H. Lu
    Pages 29-36
  6. Multiwell Plate Tools for Controlling Cellular Alignment with Grooved Topography

    • Camila Londono, John Soleas, Petra B. Lücker, Suthamathy Sathananthan, J. Stewart Aitchison, Alison P. McGuigan
    Pages 37-55
  7. Bioreactor Cultivation of Anatomically Shaped Human Bone Grafts

    • Joshua P. Temple, Keith Yeager, Sarindr Bhumiratana, Gordana Vunjak-Novakovic, Warren L. Grayson
    Pages 57-78
  8. Conjugation of Proteins to Polymer Chains to Create Multivalent Molecules

    • Anthony Conway, Dawn P. Spelke, David V. Schaffer
    Pages 95-102
  9. An Assay to Quantify Chemotactic Properties of Degradation Products from Extracellular Matrix

    • Brian M. Sicari, Li Zhang, Ricardo Londono, Stephen F. Badylak
    Pages 103-110
  10. Extracellular Matrix Mimetic Peptide Scaffolds for Neural Stem Cell Culture and Differentiation

    • Busra Mammadov, Mustafa O. Guler, Ayse B. Tekinay
    Pages 131-148
  11. Cultivation of Human Bone-Like Tissue from Pluripotent Stem Cell-Derived Osteogenic Progenitors in Perfusion Bioreactors

    • Giuseppe Maria de Peppo, Gordana Vunjak-Novakovic, Darja Marolt
    Pages 173-184
  12. Back Matter

    Pages 185-187

About this book

Biomimetics and Stem Cells: Methods and Protocols collects a series of approaches to demonstrate the role and value of biomimetics for the better understanding of stem cell behavior and the acceleration of their application in regenerative medicine. Recent advances in tissue engineering are enabling scientists to ‘‘instruct’’ stem cells toward differentiating into the right phenotypes, in the right place and at the right time. Given these advances, biomimetic environments are being designed to recapitulate, in vitro, the combinations of factors known to guide tissue development and regeneration in vivo and thereby help unlock the full potential of the stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and essential, Biomimetics and Stem Cells: Methods and Protocols focuses on the use of biomimetic systems for stem cells in order to aid in moving this vital field of study forward.

Editors and Affiliations

  • Department of Biomedical Engineering and Department of Medicine, Columbia University, New York, USA

    Gordana Vunjak-Novakovic

  • Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Biomimetics and Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Gordana Vunjak-Novakovic, Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1332-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1331-2Published: 02 July 2014

  • Softcover ISBN: 978-1-4939-4431-6Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1332-9Published: 30 June 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 187

  • Number of Illustrations: 23 b/w illustrations, 31 illustrations in colour

  • Topics: Cell Biology, Stem Cells, Regenerative Medicine/Tissue Engineering

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access