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Quantum Dots: Applications in Biology

  • Book
  • © 2014

Overview

Editors:
  1. Adriana Fontes

    1. Research Group on Biomedical Nanotechnology Biophysics and Radiobiology Department, Federal University of Pernambuco, Recife, Brazil

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    You can also search for this editor in PubMed   Google Scholar

  2. Beate Saegesser Santos

    1. Research Group on Biomedical Nanotechnology Pharmaceutical Sciences Department, Federal University of Pernambuco, Recife, Brazil

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1199)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
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  2. Introduction to Quantum Dots

    1. Front Matter

      Pages 1-1
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    2. Quantum Dots as Biophotonics Tools

      • Carlos L. Cesar
      Pages 3-9

  3. Quantum Dots’ Preparative Processes and Characterizations

    1. Front Matter

      Pages 11-11
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    2. Photoligation Combined with Zwitterion-Modified Lipoic Acid Ligands Provides Compact and Biocompatible Quantum Dots

      • Naiqian Zhan, Goutam Palui, Henry Grise, Hedi Mattoussi
      Pages 13-31

    3. Analysis of Quantum Dots and Their Conjugates by Capillary Electrophoresis with Detection of Laser-Induced Luminescence

      • Karel Klepárník, Vladimíra Datinská, Ivona Voráčová, Marcela Lišková
      Pages 33-54

    4. Advanced Procedure for Oriented Conjugation of Full-Size Antibodies with Quantum Dots

      • Kristina Brazhnik, Igor Nabiev, Alyona Sukhanova
      Pages 55-66

    5. Quantum Dot–Antibody Conjugates via Carbodiimide-Mediated Coupling for Cellular Imaging

      • Daniel Alistair East, Michael Todd, Ian James Bruce
      Pages 67-83

    6. Measuring the Hydrodynamic Radius of Quantum Dots by Fluorescence Correlation Spectroscopy

      • André A. de Thomaz, Diogo B. Almeida, Carlos L. Cesar
      Pages 85-91

    7. Quantum Dots Fluorescence Quantum Yield Measured by Thermal Lens Spectroscopy

      • Carlos Estupiñán-López, Christian Tolentino Dominguez, Paulo E. Cabral Filho, Adriana Fontes, Renato E. de Araujo
      Pages 93-101

    8. Semiquantitative Fluorescence Method for Bioconjugation Analysis

      • Aluízio G. Brasil Jr., Kilmara H. G. Carvalho, Elisa S. Leite, Adriana Fontes, Beate Saegesser Santos
      Pages 103-110

  4. Quantum Dots for Live Cells and Tissues Applications

    1. Front Matter

      Pages 111-111
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    2. Assembly, Characterization, and Delivery of Quantum Dot Labeled Biotinylated Lipid Particles

      • Valeria Sigot
      Pages 113-127

    3. Oriented Conjugation of Single-Domain Antibodies and Quantum Dots

      • Kristina Brazhnik, Igor Nabiev, Alyona Sukhanova
      Pages 129-140

    4. Functional Integration of Quantum Dot Labeled Mesenchymal Stem Cells in a Cardiac Microenvironment

      • Maria C. Collins, Philip R. Gunst, Barbara J. Muller-Borer
      Pages 141-154

    5. In Vitro Approaches to Assessing the Toxicity of Quantum Dots

      • Ryan S. McMahan, Vivian Lee, William C. Parks, Terrance J. Kavanagh, David L. Eaton
      Pages 155-163

    6. Basics for the Preparation of Quantum Dots and Their Interactions with Living Cells

      • Xiue Jiang, Jing Bai, Tiantian Wang
      Pages 165-175

  5. QDs for Small Animals Applications

    1. Front Matter

      Pages 177-177
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    2. In Vivo Approaches to Assessing the Toxicity of Quantum Dots

      • David K. Scoville, Christopher M. Schaupp, François Baneyx, Terrance J. Kavanagh
      Pages 179-190

    3. Quantum Dots for Imaging Neural Cells In Vitro and In Vivo

      • Angela O. Choi, Kevin D. Neibert, Dusica Maysinger
      Pages 191-206
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Keywords

About this book

In Quantum Dots: Applications in Biology, Second Edition, expert researchers in the field detail consolidated approaches as well as new trends in the field. Organized into five parts, the first part comprises an introduction on Quantum Dots (QDs) as fluorescent probes in Life Sciences. While the second section covers important features about QDs´ preparative processes and characterizations for their successful application as fluorophores. The third part presents main aspects related to QDs methods applied to live cells and tissues. The fourth section focuses on QDs experiments in small animals and the fifth part demonstrates the versatility of QDs in a set of FRET applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Thorough and intuitive, In Quantum Dots: Applications in Biology, Second Edition aids scientists in continuing to study QDs by providing information about methods and protocols helping to expand their research.

Editors and Affiliations

  • Research Group on Biomedical Nanotechnology Biophysics and Radiobiology Department, Federal University of Pernambuco, Recife, Brazil

    Adriana Fontes

  • Research Group on Biomedical Nanotechnology Pharmaceutical Sciences Department, Federal University of Pernambuco, Recife, Brazil

    Beate Saegesser Santos

Bibliographic Information

  • Book Title: Quantum Dots: Applications in Biology

  • Editors: Adriana Fontes, Beate Saegesser Santos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1280-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1279-7Published: 08 August 2014

  • Softcover ISBN: 978-1-4939-5415-5Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1280-3Published: 07 August 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 258

  • Number of Illustrations: 11 b/w illustrations, 65 illustrations in colour

  • Topics: Biotechnology, Nanotechnology, Biological Microscopy

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