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  • © 2014

Epidermal Cells

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols on epidermal cell research
  • Fully updated from the previous editions
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1195)

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eBook USD 89.00
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Softcover Book USD 119.99
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  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
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Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
  2. Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage

    • Igor Kogut, Dennis R. Roop, Ganna Bilousova
    Pages 1-12
  3. Analysis and Meta-analysis of Transcriptional Profiling in Human Epidermis

    • Claudia Mimoso, Ding-Dar Lee, Jiri Zavadil, Marjana Tomic-Canic, Miroslav Blumenberg
    Pages 61-97
  4. Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity

    • Lakiea J. Bailey, Vivek Choudhary, Purnima Merai, Wendy B. Bollag
    Pages 111-131
  5. Lipid Rafts and Detergent-Resistant Membranes in Epithelial Keratinocytes

    • Kathleen P. McGuinn, Mỹ G. Mahoney
    Pages 133-144
  6. Modified Methods for Growing 3-D Skin Equivalents: An Update

    • Rebecca Lamb, Carrie A. Ambler
    Pages 171-182
  7. Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter

    • Evelyne De Vuyst, Céline Charlier, Séverine Giltaire, Valérie De Glas, Catherine Lambert de Rouvroit, Yves Poumay
    Pages 191-201
  8. Human Keratinocyte Cultures in the Investigation of Early Steps of Human Papillomavirus Infection

    • Laura M. Griffin, Louis Cicchini, Tao Xu, Dohun Pyeon
    Pages 219-238
  9. Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies

    • Inês Sequeira, Emilie Legué, Suzanne Capgras, Jean-François Nicolas
    Pages 247-258

About this book

Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition. The inclusion of protocols useful for both in vitro and in vivo studies reflects many useful developments in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Dependable and easy to follow, Epidermal Cells: Methods and Protocols, Third Edition serves researchers working to accelerate the work in this vital field of study.

Editors and Affiliations

  • Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Epidermal Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1224-7

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1223-0Published: 14 July 2014

  • Softcover ISBN: 978-1-4939-5578-7Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1224-7Published: 11 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 296

  • Number of Illustrations: 116 b/w illustrations, 34 illustrations in colour

  • Topics: Cell Biology, Stem Cells, Cell Culture

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access