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  • © 2014

Mouse Genetics

Methods and Protocols

  • Includes cutting-edge methods and protocols in mouse genetic research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1194)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
  2. Mouse Genetics Techniques

    1. Front Matter

      Pages 1-1
    2. Generation of Conditional Knockout Mice

      • Kazuhito Sakamoto, Channabasavaiah B. Gurumurthy, Kay-Uwe Wagner
      Pages 21-35
    3. Generating Mouse Lines for Lineage Tracing and Knockout Studies

      • Petra Kraus, V. Sivakamasundari, Xing Xing, Thomas Lufkin
      Pages 37-62
    4. Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases

      • Susanne Feil, Jana Krauss, Martin Thunemann, Robert Feil
      Pages 113-139
    5. Intracytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice

      • Pedro N. Moreira, Lluís Montoliu
      Pages 141-156
    6. Generation of Mouse Lines Conditionally Over-expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System

      • Claudia Piovan, Foued Amari, Francesca Lovat, Qun Chen, Vincenzo Coppola
      Pages 203-224
    7. In Situ Hybridization (Both Radioactive and Nonradioactive) and Spatiotemporal Gene Expression Analysis

      • Olga Simmons, Esther M. Bolanis, Jian Wang, Simon J. Conway
      Pages 225-244
  3. Mouse Genetics in Stem Cells

    1. Front Matter

      Pages 245-245
    2. Isolation and Handling of Mouse Embryonic Fibroblasts

      • Kanika Jain, Paul J. Verma, Jun Liu
      Pages 247-252
    3. Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue

      • Pollyanna Agnes Goh, Paul J. Verma
      Pages 253-270
    4. Pdx1 (GFP/w) Mice for Isolation, Characterization, and Differentiation of Pancreatic Progenitor Cells

      • Michael D. Williams, Wilson Wong, Amanda Rixon, Sarang N. Satoor, Anandwardhan A. Hardikar, Mugdha V. Joglekar
      Pages 271-288
    5. Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development

      • Andrei M. Vacaru, Joseph Vitale, Johnathan Nieves, Margaret H. Baron
      Pages 289-312

About this book

Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.

Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.

Editors and Affiliations

  • Stem Cell Regulation and Animal Aging Section, Basic Research Laboratory,, National Cancer Institute, National Institutes of Health, Frederick, USA

    Shree Ram Singh

  • Genetically Engineered Mouse Modeling Core/Department of Molecular Virology, Immunology and Medical Genetics, Wexner Medical Center, Comprehensive Cancer Center, The Ohio State University, Columbus, USA

    Vincenzo Coppola

Bibliographic Information

  • Book Title: Mouse Genetics

  • Book Subtitle: Methods and Protocols

  • Editors: Shree Ram Singh, Vincenzo Coppola

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1215-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1214-8Published: 26 July 2014

  • Softcover ISBN: 978-1-4939-4567-2Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1215-5Published: 26 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 475

  • Number of Illustrations: 24 b/w illustrations, 68 illustrations in colour

  • Topics: Human Genetics, Animal Genetics and Genomics, Animal Models

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access