Overview
- Includes cutting-edge methods and protocols involving mast cells and basophils
- Provides step-by-step details essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1192)
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Table of contents (18 protocols)
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Front Matter
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Introduction & Historical Background
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Front Matter
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Obtaining the Cells: Purification, Culture, Counting, and Cell Lines
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Front Matter
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Whole Cells: Identification in Tissues, Signaling Pathways, Gene Silencing, and Reporters
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Front Matter
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Keywords
About this book
In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these cells from the most practical point of view. Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. Also covered are protocols for the in vitro and in vivo study of their functions. Written in the highly successful Methods in Molecular Biology format, chapters in this book contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls straight from the researchers who use the methods most.
Authoritative and easy to use, Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future research into mast cells and basophils with the goal of aiding in the quest to shed more light on these fascinating cell types.
Editors and Affiliations
Bibliographic Information
Book Title: Basophils and Mast Cells
Book Subtitle: Methods and Protocols
Editors: Bernhard F. Gibbs, Franco H. Falcone
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1173-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1172-1Published: 23 August 2014
Softcover ISBN: 978-1-4939-5409-4Published: 17 September 2016
eBook ISBN: 978-1-4939-1173-8Published: 22 August 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 251
Number of Illustrations: 11 b/w illustrations, 28 illustrations in colour
Topics: Immunology