Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1191)
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Table of contents (17 protocols)
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Front Matter
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Models: Stoichiometry, Network Generation
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Front Matter
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Thermodynamics: A Step Beyond MFA
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Front Matter
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Experiments: Design Considerations, Fermentation, Analytics
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Front Matter
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13C Fluxomics Data Processing
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Front Matter
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Examples
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Front Matter
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About this book
Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach. Metabolic flux analysis emerged from the current limitation for the prediction of metabolic fluxes from a measured inventory of the cell. Divided into convenient thematic parts, topics in this essential volume include the fundamental characteristics of the underlying networks, the application of quantitative metabolite data and thermodynamic principles to constrain the solution space for flux balance analysis (FBA), the experimental toolbox to conduct different types of flux analysis experiments, the processing of data from 13C experiments and three chapters that summarize some recent key findings. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Metabolic Flux Analysis: Methods and Protocols presents protocols that cover a range of relevant organisms currently used in the field, providing a solid basis to anybody interested in the field of metabolic flux analysis.
Editors and Affiliations
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Centre for Microbial Electrosynthesis (CEMES), Advanced Water Management Centre, University of Queensland, St. Lucia, Brisbane, Australia
Jens O. Krömer
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AIBN, University of Queensland, St. Lucia, Brisbane, Australia
Lars K. Nielsen
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Biology Department, RWTH Aachen University, Aachen, Germany
Lars M. Blank
Bibliographic Information
Book Title: Metabolic Flux Analysis
Book Subtitle: Methods and Protocols
Editors: Jens O. Krömer, Lars K. Nielsen, Lars M. Blank
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1170-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1169-1Published: 03 September 2014
Softcover ISBN: 978-1-4939-4159-9Published: 17 September 2016
eBook ISBN: 978-1-4939-1170-7Published: 01 September 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 316
Number of Illustrations: 19 b/w illustrations, 51 illustrations in colour
Topics: Biochemistry, general, Enzymology