Overview
- Includes cutting-edge methods and protocols involving B cells with suppressive functions and their immunological properties
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1190)
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Table of contents (19 protocols)
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Front Matter
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Purification and Phenotypic Characterization of B Cell Subsets
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Front Matter
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Mechanisms of Immune Suppression by B Cells
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Front Matter
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Methods for the Ex Vivo Generation/Expansion of IL-10-Producing B Cells
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Front Matter
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Study of Regulatory B Cells in Pathological Settings
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Front Matter
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Keywords
About this book
Regulatory B Cells: Methods and Protocols present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology.
Editors and Affiliations
Bibliographic Information
Book Title: Regulatory B Cells
Book Subtitle: Methods and Protocols
Editors: Gaetano Vitale, Francesca Mion
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1161-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-1160-8Published: 14 July 2014
Softcover ISBN: 978-1-4939-4677-8Published: 23 August 2016
eBook ISBN: 978-1-4939-1161-5Published: 11 July 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 292
Number of Illustrations: 34 b/w illustrations, 19 illustrations in colour
Topics: Immunology, Cell Culture