Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups. These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research.

• Cell Culture
• Gram negative bacteria
• Gram positive bacteria
• RNAi-mediated gene knockdown
• SILAC labeling of the yeast Saccharomyces cerevisiae
• SILAC technology
• Trypanosoma brucei
• autotrophic organisms
• human protein complexes
• human splicosome
• metabolic labeling
• nuclear protein-protein interactions
• protein interactomes
• quantitative phosphoproteome analyses in C. elegans
• signaling networks
• time-resolved phosphoproteomics studies

• Institute of Biology II, University of Freiburg, Freiburg, Germany

  Bettina Warscheid

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