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Hematopoietic Stem Cell Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Kevin D. Bunting

    1. Department of Pediatrics, Aflac Cancer and Blood Disorders Center Emory University School of Medicine, Atlanta, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Cheng-Kui Qu

    1. Department of Pediatrics, Emory University School of Medicine Aflac Cancer & Blood Disorders Ctr, Atlanta, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1185)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Hematopoietic Stem Cell Landscape

      • Kevin D. Bunting, Cheng-Kui Qu
      Pages 3-6

  3. Stem Cell Enrichment and Heterogeneity

    1. Front Matter

      Pages 7-7
      Download chapter PDF

    2. Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow

      • Huimin Cao, Brenda Williams, Susan K. Nilsson
      Pages 9-20

    3. Single-Cell PCR Profiling of Gene Expression in Hematopoiesis

      • José Teles, Tariq Enver, Cristina Pina
      Pages 21-42

    4. Hematopoietic Stem and Progenitor Cell Mobilization in Mice

      • Jonathan Hoggatt, Tiffany A. Tate, Louis M. Pelus
      Pages 43-64

    5. Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells

      • Brahmananda Reddy Chitteti, Edward F. Srour
      Pages 65-78

    6. Flow Cytometric Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells

      • Lei Dong, Cheng-Kui Qu
      Pages 79-87

  4. Molecular and Cellular Characterization

    1. Front Matter

      Pages 89-89
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    2. Gene Expression Profiling of Hematopoietic Stem Cells (HSCs)

      • Nalini Raghavachari
      Pages 91-119

    3. Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells

      • Wenhuo Hu, Christopher Y. Park
      Pages 121-140

    4. DNA Methylation Profiling of Hematopoietic Stem Cells

      • Amber Hogart Begtrup
      Pages 141-153

    5. Metabolic Characterization of Hematopoietic Stem Cells

      • Fatih Kocabas, Junke Zheng, Chengcheng Zhang, Hesham A. Sadek
      Pages 155-164

    6. Nanoproteomic Assays on Hematopoietic Stem Cells

      • Heath L. Bradley, Himalee Sabnis, Deborah Pritchett, Kevin D. Bunting
      Pages 165-177

  5. In Vitro Assays and Differentiation

    1. Front Matter

      Pages 179-179
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    2. Hematopoietic Differentiation of Pluripotent Stem Cells in Culture

      • Jason A. Mills, Prasuna Paluru, Mitchell J. Weiss, Paul Gadue, Deborah L. French
      Pages 181-194

    3. Ex Vivo Assays to Study Self-Renewal, Long-Term Expansion, and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells

      • Pallavi Sontakke, Marco Carretta, Marta Capala, Hein Schepers, Jan Jacob Schuringa
      Pages 195-210

    4. Ex Vivo Expansion of Murine and Human Hematopoietic Stem Cells

      • Phuong L. Doan, John P. Chute
      Pages 211-221

    5. Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level

      • Byungwook Ahn, Zhengqi Wang, David R. Archer, Wilbur A. Lam
      Pages 223-233
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Keywords

About this book

Updating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protocols from leading stem cell researchers. This in-depth volume presents a clear view of the landscape of assays available to the stem cell researcher working in the growing hematopoietic stem cell (HSC) field. A robust and active field, it is supported by an abundance of innovative mouse models and molecular tools for analysis of phenotypes and functions in mouse and human cells. Understanding more about hematopoietic stem cell biology is integral if these versatile cells are to be applied effectively to treat and cure a wide range of blood diseases. An introductory chapter puts the major contributions of the book into the proper perspective. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Third Edition is a much needed technical resource in the critically important field of hematopoietic stem cell investigation.

Editors and Affiliations

  • Department of Pediatrics, Aflac Cancer and Blood Disorders Center Emory University School of Medicine, Atlanta, USA

    Kevin D. Bunting

  • Department of Pediatrics, Emory University School of Medicine Aflac Cancer & Blood Disorders Ctr, Atlanta, USA

    Cheng-Kui Qu

Bibliographic Information

  • Book Title: Hematopoietic Stem Cell Protocols

  • Editors: Kevin D. Bunting, Cheng-Kui Qu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1133-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1132-5Published: 26 July 2014

  • Softcover ISBN: 978-1-4939-4757-7Published: 17 September 2016

  • eBook ISBN: 978-1-4939-1133-2Published: 25 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 364

  • Number of Illustrations: 10 b/w illustrations, 42 illustrations in colour

  • Topics: Cell Biology, Stem Cells

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