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  • Book
  • © 2014

Cardiac Tissue Engineering

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1181)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
  2. Second Generation Codon Optimized Minicircle (CoMiC) for Nonviral Reprogramming of Human Adult Fibroblasts

    • Sebastian Diecke, Leszek Lisowski, Nigel G. Kooreman, Joseph C. Wu
    Pages 1-13
  3. Preparation and Characterization of Circulating Angiogenic Cells for Tissue Engineering Applications

    • Aleksandra Ostojic, Suzanne Crowe, Brian McNeill, Marc Ruel, Erik J. Suuronen
    Pages 27-38
  4. Synthesis of Aliphatic Polyester Hydrogel for Cardiac Tissue Engineering

    • Sanjiv Dhingra, Richard D. Weisel, Ren-Ke Li
    Pages 51-59
  5. Fabrication of PEGylated Fibrinogen: A Versatile Injectable Hydrogel Biomaterial

    • Iris Mironi-Harpaz, Alexandra Berdichevski, Dror Seliktar
    Pages 61-68
  6. Natural Cardiac Extracellular Matrix Hydrogels for Cultivation of Human Stem Cell-Derived Cardiomyocytes

    • Donald O. Freytes, John D. O’Neill, Yi Duan-Arnold, Emily A. Wrona, Gordana Vunjak-Novakovic
    Pages 69-81
  7. Shrink-Induced Biomimetic Wrinkled Substrates for Functional Cardiac Cell Alignment and Culture

    • Nicole Mendoza, Roger Tu, Aaron Chen, Eugene Lee, Michelle Khine
    Pages 97-108
  8. Injectable ECM Scaffolds for Cardiac Repair

    • Todd D. Johnson, Rebecca L. Braden, Karen L. Christman
    Pages 109-120
  9. Generation of Strip-Format Fibrin-Based Engineered Heart Tissue (EHT)

    • Sebastian Schaaf, Alexandra Eder, Ingra Vollert, Andrea Stöhr, Arne Hansen, Thomas Eschenhagen
    Pages 121-129
  10. Cell Tri-Culture for Cardiac Vascularization

    • Ayelet Lesman, Lior Gepstein, Shulamit Levenberg
    Pages 131-137
  11. Cell Sheet Technology for Cardiac Tissue Engineering

    • Yuji Haraguchi, Tatsuya Shimizu, Katsuhisa Matsuura, Hidekazu Sekine, Nobuyuki Tanaka, Kenjiro Tadakuma et al.
    Pages 139-155
  12. Design and Fabrication of Biological Wires

    • Jason W. Miklas, Sara S. Nunes, Boyang Zhang, Milica Radisic
    Pages 157-165
  13. Collagen-Based Engineered Heart Muscle

    • Malte Tiburcy, Tim Meyer, Poh Loong Soong, Wolfram-Hubertus Zimmermann
    Pages 167-176
  14. Patch-Clamp Technique in ESC-Derived Cardiomyocytes

    • Jie Liu, Peter H. Backx
    Pages 203-214
  15. Optogenetic Control of Cardiomyocytes via Viral Delivery

    • Christina M. Ambrosi, Emilia Entcheva
    Pages 215-228

About this book

Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.

Editors and Affiliations

  • Department Chemical Engineering&Applied Chemistry, University of Toronto, Toronto, Canada

    Milica Radisic

  • Department of Biomedical Engineering, Tufts University, Medford, USA

    Lauren D. Black III

Bibliographic Information

  • Book Title: Cardiac Tissue Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Milica Radisic, Lauren D. Black III

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1047-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1046-5Published: 29 July 2014

  • Softcover ISBN: 978-1-4939-4613-6Published: 24 September 2016

  • eBook ISBN: 978-1-4939-1047-2Published: 28 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 266

  • Number of Illustrations: 17 b/w illustrations, 60 illustrations in colour

  • Topics: Biotechnology, Regenerative Medicine/Tissue Engineering

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access