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Eosinophils

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Garry M. Walsh

    1. Division of Applied Medicine, University of Aberdeen Institute of Medical Sciences, Aberdeen, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1178)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Eosinophil Overview: Structure, Biological Properties, and Key Functions

    • Paige Lacy, Helene F. Rosenberg, Garry M. Walsh
    Pages 1-12

  3. Eosinophil Purification from Peripheral Blood

    • Praveen Akuthota, Kelsey Capron, Peter F. Weller
    Pages 13-20

  4. Eosinophil Purification from Human Bone Marrow

    • Tina W. Wong, Diane F. Jelinek
    Pages 21-27

  5. CD34+ Eosinophil-Lineage-Committed Cells in the Mouse Lung

    • Apostolos Bossios, Madeleine Rådinger
    Pages 29-43

  6. Eosinophil Cell Lines

    • Kenji Ishihara
    Pages 45-51

  7. Cell Signalling During Human Eosinophil Differentiation

    • Miranda Buitenhuis
    Pages 53-70

  8. Eosinophil Intracellular Signalling: Apoptosis

    • Pinja Ilmarinen, Eeva Moilanen, Hannu Kankaanranta
    Pages 71-80

  9. Identification of Human Eosinophils in Whole Blood by Flow Cytometry

    • Caroline Ethier, Paige Lacy, Francis Davoine
    Pages 81-92

  10. Isolation and Functional Assessment of Eosinophil Crystalloid Granules

    • Renata Baptista-dos-Reis, Valdirene S. Muniz, Josiane S. Neves
    Pages 93-100

  11. Eosinophil Chemotaxis

    • Gordon Dent
    Pages 101-110

  12. Eosinophil Shape Change and Secretion

    • Lian Willetts, Sergei I. Ochkur, Elizabeth A. Jacobsen, James J. Lee, Paige Lacy
    Pages 111-128

  13. Human Eosinophil Adhesion and Receptor Expression

    • Colleen S. Curran
    Pages 129-141

  14. Adhesion of Eosinophils to Endothelial Cells or Substrates Under Flow Conditions

    • Viktoria Konya, Miriam Peinhaupt, Akos Heinemann
    Pages 143-156

  15. Human Eosinophil Transmigration

    • Stanislawa Bazan-Socha, Joanna Zuk, Bogdan Jakiela, Jacek Musial
    Pages 157-164

  16. Measurement of Eosinophil Kinetics in Healthy Volunteers

    • Neda Farahi, Chrystalla Loutsios, Rosalind P. Simmonds, Linsey Porter, Daniel Gillett, Sarah Heard et al.
    Pages 165-176

  17. Assays of Eosinophil Apoptosis and Phagocytic Uptake

    • David A. Dorward, Sidharth Sharma, Ana L. Alessandri, Adriano G. Rossi, Christopher D. Lucas
    Pages 177-195

  18. Clinical Measurement of Eosinophil Numbers in Eosinophilic Conjunctivitis

    • Osmo Kari, K. Matti Saari
    Pages 197-202

  19. Qualitative and Quantitative Studies of Eosinophils in Parasitic Infections

    • Masataka Korenaga, Fabrizio Bruschi
    Pages 203-213

  20. Interactions of Eosinophils with Nerves

    • Quinn R. Roth-Carter, David B. Jacoby, Zhenying Nie
    Pages 215-229
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Keywords

About this book

In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry.​ Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Eosinophil: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and for further examination of this intriguing cell.

Editors and Affiliations

  • Division of Applied Medicine, University of Aberdeen Institute of Medical Sciences, Aberdeen, United Kingdom

    Garry M. Walsh

Bibliographic Information

  • Book Title: Eosinophils

  • Book Subtitle: Methods and Protocols

  • Editors: Garry M. Walsh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1016-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-1015-1Published: 03 July 2014

  • Softcover ISBN: 978-1-4939-5509-1Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1016-8Published: 01 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 324

  • Number of Illustrations: 25 b/w illustrations, 39 illustrations in colour

  • Topics: Immunology

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