Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1158)
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Table of contents (23 protocols)
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Front Matter
Keywords
- RNA-protein interaction
- cellular redox state
- clock-controlled parameters
- computational biology
- flowering time control
- in vivo DNA-protein interaction
- luciferase reporters
- posttranslational modification
- protein levels
- protein-protein interaction
- small RNA binding sites
- small RNA levels
- transcription factor binding sites
About this book
Plant Circadian Networks: Methods and Protocols provides a collection of protocols to investigate clock-controlled parameters including transcript and small RNA levels, promoter activity using luciferase reporters, protein levels and posttranslational modification, protein-protein interaction, in vivo DNA-protein interaction and RNA-protein interaction, cellular redox state, Ca2+ levels, and innate immune responses. Furthermore, the use of bioinformatics resources is described to evaluate high throughput data sets and to integrate the data into an overarching picture of circadian networks in the cell.
Additional chapters focus on seasonal processes like flowering time control, and techniques on trees, moss and algae. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Circadian Networks: Methods and Protocols is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general.
Editors and Affiliations
Bibliographic Information
Book Title: Plant Circadian Networks
Book Subtitle: Methods and Protocols
Editors: Dorothee Staiger
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-0700-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, New York 2014
Hardcover ISBN: 978-1-4939-0699-4Published: 03 May 2014
Softcover ISBN: 978-1-4939-4587-0Published: 23 August 2016
eBook ISBN: 978-1-4939-0700-7Published: 02 May 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 361
Number of Illustrations: 30 b/w illustrations, 47 illustrations in colour
Topics: Plant Sciences