Editors:
- Includes cutting-edge methods and protocols involving a wide array of proteome studies
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1156)
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Table of contents (31 protocols)
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Front Matter
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Introduction
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Front Matter
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In Vitro Stable Isotope Labeling
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Front Matter
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Label-Free Proteomics
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Front Matter
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About this book
Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified and characterized in a high throughput manner. Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species.
Editors and Affiliations
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Ludwig-Maximilians-University, Munich, Germany
Daniel Martins-de-Souza
Bibliographic Information
Book Title: Shotgun Proteomics
Book Subtitle: Methods and Protocols
Editors: Daniel Martins-de-Souza
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-0685-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-0684-0Published: 03 May 2014
Softcover ISBN: 978-1-4939-4695-2Published: 30 April 2017
eBook ISBN: 978-1-4939-0685-7Published: 02 May 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 492
Number of Illustrations: 33 b/w illustrations, 48 illustrations in colour
Topics: Protein Science, Proteomics