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  • © 2014

Plant Isoprenoids

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1153)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. Plant Isoprenoids: A General Overview

    • Manuel Rodríguez-Concepción
    Pages 1-5
  3. Measurement of Core Enzyme Activities

    1. Front Matter

      Pages 7-7
    2. Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants

      • Narciso Campos, Montserrat Arró, Albert Ferrer, Albert Boronat
      Pages 21-40
    3. Farnesyl Diphosphate Synthase Assay

      • Montserrat Arró, David Manzano, Albert Ferrer
      Pages 41-53
  4. Targeted Analysis of Isoprenoid Metabolites

    1. Front Matter

      Pages 55-55
    2. Analysis of Carotenoids and Tocopherols in Plant Matrices and Assessment of Their In Vitro Antioxidant Capacity

      • Antonio J. Meléndez-Martínez, Carla M. Stinco, Paula Mapelli Brahm, Isabel M. Vicario
      Pages 77-97
    3. Analysis of Plant Polyisoprenoids

      • Katarzyna Gawarecka, Ewa Swiezewska
      Pages 135-147
    4. Analysis of Diterpenes and Triterpenes from Plant Foliage and Roots

      • Qiang Wang, Reza Sohrabi, Dorothea Tholl
      Pages 149-159
  5. Isoprenoid Profiling in Specialized Organs

    1. Front Matter

      Pages 187-187
    2. Isoprenoid and Metabolite Profiling of Plant Trichomes

      • Gerd U. Balcke, Stefan Bennewitz, Sebastian Zabel, Alain Tissier
      Pages 189-202
    3. Prenylquinone Profiling in Whole Leaves and Chloroplast Subfractions

      • Felix Kessler, Gaetan Glauser
      Pages 213-226

About this book

Plant Isoprenoids: Methods and Protocols is a collection of detailed techniques that will be a useful tool for a wide range of plant biologists, as well as for scientists of other fields interested in plant isoprenoids. Isoprenoids are an incredibly diverse family and they participate in a large variety of processes. Divided into four convenient sections to better cover strategic areas in plant isoprenoid research, topics include measurement of core enzyme activities involved in the production of isoprenoid precursors, targeted analysis of major groups of isoprenoid metabolites, isoprenoid profiling in specialized organs such as trichomes and oil glands as well as genetic, pharmacological and bioinformatic tools that are particularly useful for plant molecular biologists. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Plant Isoprenoids: Methods and Protocols will serve as an excellent reference material that can be adapted to develop customized methods for different needs making the world of plant isoprenoids more accessible for all researchers.

Editors and Affiliations

  • Centre for Research in Agricultural Genomic (CRAG), CSIC-IRTA-UAB-UB, Barcelona, Spain

    Manuel Rodríguez-Concepción

Bibliographic Information

  • Book Title: Plant Isoprenoids

  • Book Subtitle: Methods and Protocols

  • Editors: Manuel Rodríguez-Concepción

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0606-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0605-5Published: 29 April 2014

  • Softcover ISBN: 978-1-4939-5391-2Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0606-2Published: 28 April 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 303

  • Number of Illustrations: 41 b/w illustrations, 12 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access