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  • © 2014

Photoswitching Proteins

Methods and Protocols

  • Presents current up-to-date methodologies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1148)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
  2. Photoinduced Damage Resulting from Fluorescence Imaging of Live Cells

    • Matthew K. Daddysman, Michael A. Tycon, Christopher J. Fecko
    Pages 1-17
  3. Optochemical Activation of Kinase Function in Live Cells

    • Andrei V. Karginov, Klaus M. Hahn, Alexander Deiters
    Pages 31-43
  4. Photoswitching of Cell Surface Receptors Using Tethered Ligands

    • Andreas Reiner, Ehud Y. Isacoff
    Pages 45-68
  5. Photocontrol of AMPA Receptors with a Photochromic Ligand

    • Martin Sumser, Philipp Stawski
    Pages 69-76
  6. Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins

    • Lauren R. Polstein, Charles A. Gersbach
    Pages 89-107
  7. A Roadmap to Applying Optogenetics in Neuroscience

    • Consuelo Fois, Pierre-Hugues Prouvot, Albrecht Stroh
    Pages 129-147
  8. Salvaging Ruins: Reverting Blind Retinas into Functional Visual Sensors

    • Marion Mutter, Natalia Swietek, Thomas A. Münch
    Pages 149-160
  9. Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity

    • Wagner Steuer Costa, Jana Liewald, Alexander Gottschalk
    Pages 161-175
  10. Structural Basis of Photoswitching in Fluorescent Proteins

    • Chenxi Duan, Virgile Adam, Martin Byrdin, Dominique Bourgeois
    Pages 177-202
  11. Using Photoactivatable GFP to Track Axonal Transport Kinetics

    • Archan Ganguly, Subhojit Roy
    Pages 203-215
  12. In Vivo Cell Tracking Using PhOTO Zebrafish

    • William P. Dempsey, Hanyu Qin, Periklis Pantazis
    Pages 217-228
  13. Photoactivatable Fluorescent Proteins for Super-resolution Microscopy

    • Yuji Ishitsuka, Karin Nienhaus, G. Ulrich Nienhaus
    Pages 239-260
  14. pcSOFI as a Smart Label-Based Superresolution Microscopy Technique

    • Benjamien Moeyaert, Peter Dedecker
    Pages 261-276
  15. Back Matter

    Pages 277-278

About this book

Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.

Editors and Affiliations

  • Institute of Anatomy and Cell Biology, University of Heidelberg, Heidelberg, Germany

    Sidney Cambridge

Bibliographic Information

  • Book Title: Photoswitching Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Sidney Cambridge

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0470-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-4939-0469-3Published: 10 April 2014

  • Softcover ISBN: 978-1-4939-5435-3Published: 17 September 2016

  • eBook ISBN: 978-1-4939-0470-9Published: 09 April 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 278

  • Number of Illustrations: 53 b/w illustrations, 5 illustrations in colour

  • Topics: Protein Science, Biological Microscopy, Neurobiology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access