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Herpes Simplex Virus

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Russell J. Diefenbach

    1. Centre for Virus Research, Westmead Millennium Institute, Westmead, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Cornel Fraefel

    1. Institute of Virology, University of Zürich, Switzerland

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of the complex interactions between herpes viruses and the host
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1144)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. HSV-1 Biology and Life Cycle

    • Roger D. Everett
    Pages 1-17

  3. Herpes Simplex Virus Growth, Preparation, and Assay

    • Peggy Marconi, Roberto Manservigi
    Pages 19-29

  4. Isolation of Herpes Simplex Virus Nucleocapsid DNA

    • Moriah Szpara
    Pages 31-41

  5. Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes

    • Claus-Henning Nagel, Anja Pohlmann, Beate Sodeik
    Pages 43-62

  6. Engineering HSV-1 Vectors for Gene Therapy

    • William F. Goins, Shaohua Huang, Justus B. Cohen, Joseph C. Glorioso
    Pages 63-79

  7. Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors

    • Matias E. Melendez, Cornel Fraefel, Alberto L. Epstein
    Pages 81-98

  8. HSV-1 Amplicon Vectors as Genetic Vaccines

    • Andrea S. Laimbacher, Cornel Fraefel
    Pages 99-115

  9. Modification of HSV-1 to an Oncolytic Virus

    • Hiroshi Nakashima, E. Antonio Chiocca
    Pages 117-127

  10. Herpes Simplex Virus Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo

    • Nancy M. Sawtell, Richard L. Thompson
    Pages 129-147

  11. Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals

    • Andreas Sauerbrei, Kathrin Bohn
    Pages 149-165

  12. Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation

    • Ju Youn Kim, Lora A. Shiflett, Jessica A. Linderman, Ian Mohr, Angus C. Wilson
    Pages 167-179

  13. Characterization of Extracellular HSV-1 Virions by Proteomics

    • Roger Lippé
    Pages 181-190

  14. A Precipitation-Based Assay to Analyze Interactions of Viral Particles with Cytosolic Host Factors

    • Kerstin Radtke, Fenja Anderson, Beate Sodeik
    Pages 191-208

  15. Affinity Purification Combined with Mass Spectrometry to Identify Herpes Simplex Virus Protein–Protein Interactions

    • David G. Meckes Jr.
    Pages 209-222

  16. Preparation of Herpes Simplex Virus-Infected Primary Neurons for Transmission Electron Microscopy

    • Monica Miranda-Saksena, Ross Boadle, Anthony L. Cunningham
    Pages 223-234

  17. Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication

    • Michael Seyffert, Anna Paula de Oliveira, Cornel Fraefel, Rebecca Vogel
    Pages 235-247

  18. Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination

    • Samuel D. Stampfer, Ekaterina E. Heldwein
    Pages 249-263

  19. Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components

    • Ryan H. Rochat, Corey W. Hecksel, Wah Chiu
    Pages 265-281

  20. In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells

    • Jolene M. Draper, Graham S. Stephenson, Jennifer H. LaVail
    Pages 283-292
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Keywords

About this book

Herpes Simplex Virus: Methods and Protocols provides a wide collection of protocols employed in various levels of herpes virus research, including basic protocols on growing viruses in cell culture and cloning, manipulating and preparing viral DNA. Other chapters describe approaches to design and apply HSV-1 vectors for vaccination, cancer and gene therapy or to study specific aspects of HSV-1 biology such as latency, intracellular transport and protein-protein interaction. Procedures for structural analyses, microscopy, proteomics and testing of antivirals are included as well. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Herpes Simplex Virus: Methods and Protocols will aid new researchers in the field of herpes virology as well as those experienced investigators wishing to embark on new techniques.

Editors and Affiliations

  • Centre for Virus Research, Westmead Millennium Institute, Westmead, Australia

    Russell J. Diefenbach

  • Institute of Virology, University of Zürich, Switzerland

    Cornel Fraefel

Bibliographic Information

  • Book Title: Herpes Simplex Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Russell J. Diefenbach, Cornel Fraefel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0428-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0427-3Published: 27 March 2014

  • Softcover ISBN: 978-1-4939-5407-0Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0428-0Published: 26 March 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 331

  • Number of Illustrations: 17 b/w illustrations, 28 illustrations in colour

  • Topics: Infectious Diseases, Virology

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