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  • © 2014

Arthritis Research

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1142)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
  2. Monitoring Multifunctionality of Immune-Exhausted CD8 T Cells in Cancer Patients

    • Shingo Eikawa, Shusaku Mizukami, Heiichiro Udono
    Pages 11-17
  3. In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets

    • Yumi Miyazaki, Shunichi Shiozawa
    Pages 49-53
  4. Characterization of MicroRNAs and Their Targets

    • Ghada Alsaleh, Jacques-Eric Gottenberg
    Pages 55-63
  5. Basic Techniques for Studies of iNKT Cells and MAIT Cells

    • Asako Chiba, Sachiko Miyake
    Pages 75-84
  6. Genome-Wide Genetic Study in Autoimmune Disease-Prone Mice

    • Masaomi Obata, Mareki Ohtsuji, Yukiyasu Iida, Toshikazu Shirai, Sachiko Hirose, Hioroyuki Nishimura
    Pages 111-141
  7. Bayesian Systems-Based Genetic Association Analysis with Effect Strength Estimation and Omic Wide Interpretation: A Case Study in Rheumatoid Arthritis

    • Gábor Hullám, András Gézsi, András Millinghoffer, Péter Sárközy, Bence Bolgár, Sanjeev K. Srivastava et al.
    Pages 143-176
  8. Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy

    • Antti Isomäki, Tarvo Sillat, Mari Ainola, Mikko Liljeström, Yrjö T. Konttinen, Mika Hukkanen
    Pages 189-201
  9. Back Matter

    Pages 203-204

About this book

Arthritis Research: Methods and Protocols, Second Edition expands upon the first edition to present new and current techniques for the research of arthritis and related conditions. A compendium of leaders in the field contribute chapters that cover practical research methods such as the intravital multiphoton microscopy technique, techniques for evaluating exhausted CD8 T cell and for studying nucleic acid sensors and their effects, methods for in vivo tetracycline-controlled transgenic mice and T cell receptor transgenic mice, protocols to detect V(D)J recombination products and microRNA, and the technique to make bleomycin-induced dermal fibrosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Arthritis Research: Methods and Protocols, Second Edition will serve both professionals and novices with state-of-the-art techniques pertaining to this fascinating research field.

Reviews

From the book reviews:

“This book offers insights and protocols regarding 16 assays used by scientists studying the workings of the immune system, particularly with respect to autoimmunity. … It is intended for scientists who are interested in reviewing the theory behind a technique and/or tips for conducting it. Clinicians trying to gain a better understanding of scientific techniques as they are appraising the primary literature in their field also could presumably use it.” (Tiphanie Vogel, Doody’s Book Reviews, September, 2014)

Editors and Affiliations

  • Department of Rheumatology, Kyushu University Beppu Hospital, Beppu, Japan

    Shunichi Shiozawa

Bibliographic Information

  • Book Title: Arthritis Research

  • Book Subtitle: Methods and Protocols

  • Editors: Shunichi Shiozawa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0404-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0403-7Published: 05 April 2014

  • Softcover ISBN: 978-1-4939-5393-6Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0404-4Published: 04 April 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 204

  • Number of Illustrations: 34 b/w illustrations, 31 illustrations in colour

  • Topics: Molecular Medicine, Orthopedics, Rheumatology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access