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Caspases,Paracaspases, and Metacaspases

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Peter V. Bozhkov

    1. Dept of Plant Biology & Forest Genetics, Swedish Univ of Agricultural Sciences Linnean Center for Plant Biology, Uppsala, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. Guy Salvesen

    1. Sanford-Burnham Medical Research Inst. Apoptosis & Cell Death Research Prgm, La Jolla, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the expert
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1133)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
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  2. Caspases

    1. Front Matter

      Pages 1-1
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    2. General In Vitro Caspase Assay Procedures

      • Dave Boucher, Catherine Duclos, Jean-Bernard Denault
      Pages 3-39

    3. Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity

      • Marcin PorÄ™ba, Aleksandra Szalek, Paulina Kasperkiewicz, Marcin DrÄ…g
      Pages 41-59

    4. Global Identification of Caspase Substrates Using PROTOMAP (Protein Topography and Migration Analysis Platform)

      • Melissa M. Dix, Gabriel M. Simon, Benjamin F. Cravatt
      Pages 61-70

    5. Caspase-2 Protocols

      • Loretta Dorstyn, Sharad Kumar
      Pages 71-87

    6. Caspase-14 Protocols

      • Mami Yamamoto-Tanaka, Toshihiko Hibino
      Pages 89-100

    7. Caspase Protocols in Caenorhabditis elegans

      • Eui Seung Lee, Ding Xue
      Pages 101-108

    8. Detecting Caspase Activity in Drosophila Larval Imaginal Discs

      • Caitlin E. Fogarty, Andreas Bergmann
      Pages 109-117

    9. Methods for the Study of Caspase Activation in the Xenopus laevis Oocyte and Egg Extract

      • Francis McCoy, Rashid Darbandi, Leta K. Nutt
      Pages 119-140

    10. Caspase Protocols in Mice

      • Varsha Kaushal, Christian Herzog, Randy S. Haun, Gur P. Kaushal
      Pages 141-154

    11. Measurement of Caspase Activation in Mammalian Cell Cultures

      • Magnus Olsson, Boris Zhivotovsky
      Pages 155-173

  3. Paracaspases and Metacaspases

    1. Front Matter

      Pages 175-175
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    2. Detection and Measurement of Paracaspase MALT1 Activity

      • Stephan Hailfinger, Christiane Pelzer, Margot Thome
      Pages 177-188

    3. Leishmania Metacaspase: An Arginine-Specific Peptidase

      • Ricardo Martin, Iveth Gonzalez, Nicolas Fasel
      Pages 189-202

    4. Purification, Characterization, and Crystallization of Trypanosoma Metacaspases

      • Karen McLuskey, Catherine X. Moss, Jeremy C. Mottram
      Pages 203-221

    5. Monitoring the Proteostasis Function of the Saccharomyces cerevisiae Metacaspase Yca1

      • Amit Shrestha, Robin E. C. Lee, Lynn A. Megeney
      Pages 223-235

    6. Plant Metacaspase Activation and Activity

      • Elena A. Minina, Simon Stael, Frank Van Breusegem, Peter V. Bozhkov
      Pages 237-253

    7. Preparation of Arabidopsis thaliana Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC

      • Liana Tsiatsiani, Simon Stael, Petra Van Damme, Frank Van Breusegem, Kris Gevaert
      Pages 255-261

  4. Back Matter

    Pages 263-266
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Keywords

About this book

Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals. Broken into two parts, the first part focuses on methods to measure, detect, and inhibit activation and activity of a subset of or specific caspases in vitro and in several model systems and organisms, primarily in the context of programmed cell death. The second part of the book provides experimental protocols for purification and in vitro and in vivo analysis of yeast, protozoan and plant metacaspases, as well as of a human paracaspase MALT1. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and practical, Caspases, Paracaspases, and Metacaspacses: Methods and Protocols seeks to aid scientists easy-to-follow techniques.

Editors and Affiliations

  • Dept of Plant Biology & Forest Genetics, Swedish Univ of Agricultural Sciences Linnean Center for Plant Biology, Uppsala, Sweden

    Peter V. Bozhkov

  • Sanford-Burnham Medical Research Inst. Apoptosis & Cell Death Research Prgm, La Jolla, USA

    Guy Salvesen

Bibliographic Information

  • Book Title: Caspases,Paracaspases, and Metacaspases

  • Book Subtitle: Methods and Protocols

  • Editors: Peter V. Bozhkov, Guy Salvesen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0357-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0356-6Published: 25 February 2014

  • Softcover ISBN: 978-1-4939-5405-6Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0357-3Published: 08 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 266

  • Number of Illustrations: 32 b/w illustrations, 34 illustrations in colour

  • Topics: Protein Science

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