The frozen-hydrated specimen is the principal element that unifies the subject of low temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com mon fact and theory upon which their work must be based. Low-Temperature Micros copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light optical microscopes.

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Authors and Affiliations

University of Cambridge, Cambridge, England

Patrick Echlin

Bibliographic Information

Book Title: Low-Temperature Microscopy and Analysis
Authors: Patrick Echlin
DOI: https://doi.org/10.1007/978-1-4899-2302-8
Publisher: Springer New York, NY
eBook Packages: Springer Book Archive
Copyright Information: Springer Science+Business Media New York 1992
Hardcover ISBN: 978-0-306-43984-1Published: 31 March 1992
Softcover ISBN: 978-1-4899-2304-2Published: 18 June 2013
eBook ISBN: 978-1-4899-2302-8Published: 11 November 2013
Edition Number: 1
Number of Pages: XXIV, 540
Topics: Developmental Biology

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Table of contents (13 chapters)

Front Matter

Back Matter

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