Electrochemical Sensors in Immunological Analysis

1. Front Matter

   Pages i-xi

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2. On the Direct Immunochemical Potentiometric Signal

   • Michael Thompson, Joseph S. Tauskela, Ulrich J. Krull

   Pages 1-18

3. Selective Antibody Responsive Membrane Electrodes

   • M. Y. Keating

   Pages 19-34

4. Electroimmunoassay of PGE2: An Antibody-Sensitive Electrode Based Competitive Protein-Binding Assay

   • George R. Connell, Kenton M. Sanders

   Pages 35-45

5. Immuno-Potentiometric Sensors with Antigen Coated Membrane

   • Shuichi Suzuki, Masuo Aizawa

   Pages 47-59

6. The Opsonized Electrode

   • H. Allen O. Hill, Nicholas J. Walton

   Pages 61-75

7. Homogeneous Voltammetric Immunoassay of Haptens

   • Sam A. McClintock, William C. Purdy

   Pages 77-85

8. Immunoassay by Differential Pulse Polarography and Anodic Stripping Voltammetry

   • Matthew J. Doyle, Kenneth R. Wehmeyer, William R. Heineman, H. Brian Halsall

   Pages 87-102

9. Separation-Free (Homogeneous) Amperometric Immunoassay Using an Apoenzyme as the Label

   • That T. Ngo

   Pages 103-115

10. Digoxin Homogeneous Enzyme Immunoassay with Amperometric Detection

    • D. Scott Wright, H. Brian Halsall, William R. Heineman

    Pages 117-130

11. Antibody to an Enzyme as the Modulator in Separation-Free Enzyme Immunoassays with Electrochemical Sensor

    • T. T. Ngo

    Pages 131-143

12. Immunochemical Determination of Creatine Kinase Isoenzyme-MB

    • Shia S. Kuan, George G. Guilbault

    Pages 145-165

13. Immunosensor Based on Labeled Liposome Entrapped-Enzymes

    • Makoto Haga

    Pages 167-177

14. Measurement of Complement Fixation with Ion Selective Membrane Electrodes

    • Paul D’Orazio

    Pages 179-194

15. Antibody-Antigen Precipitin Reaction Monitoring with an Ion Selective Electrode

    • Peter W. Alexander

    Pages 195-202

16. Enzyme-Linked Immunoassay with a Fluoride Ion Selective Electrode

    • Peter W. Alexander

    Pages 203-210

17. Heterogeneous Potentiometric Enzyme Immunoassay for Antigens and Haptens with Iodide Selective Electrode

    • Jean-Louis Boitieux, Gerard Desmet, Daniel Thomas

    Pages 211-237

18. Enzyme Immunoassay Using the Ammonia Gas-Sensing Electrode

    • Carl R. Gebauer

    Pages 239-255

19. Homogeneous and Heterogeneous Enzyme Immunoassays Monitored with Carbon Dioxide Sensing Membrane Electrodes

    • C. A. Broyles, G. A. Rechnitz

    Pages 257-268

20. Enzyme-Linked Immunosorbent Assays Using Oxygen-Sensing Electrode

    • Masuo Aizawa

    Pages 269-278

The development of radioimmunoassay (RIA) by R.S. Yalow and S.A. Berson in 1959 opens up a new avenue in ultra­ sensitive analysis of trace substances in complex biological systems. In recognition of the enormous contributions of RIA to basic research in biology and to routine clinical tests in laboratory medicine, R.S. Yalow, the co-developer of RIA, was awarded, in 1977, the Nobel Prize for Medicine and Physiology. The basic principle of RIA is elegantly simple. It is based on a specific, competitive binding reaction between the analyte and the radio-labeled analog of the analyte for the specific antibody raised to the analyte. The combination of high specificity and affinity of an antibody molecule makes it a very versatile analytical reagent capable of reacting specifically with analytes at a very low concentration in a complex solution such as serum. The sensitivity of RIA is provided by using a radioactive tracer.

• Antigen
• enzymes
• laboratory medicine
• physiology
• protein
• reaction

• University of California, Irvine, Irvine, USA

  T. T. Ngo

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