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  • Book
  • © 1993

Molecular Interactions in Bioseparations

Editors:

  1. That T. Ngo

    1. Department of Developmental and Cell Biology, University of California, Irvine, USA

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Table of contents (35 chapters)

  1. Front Matter

    Pages i-xvii
    PDF

  2. General Introduction

    1. Front Matter

      Pages 1-1
      PDF

    2. Affinity Chromatography

      • Indu Parikh, Pedro Cuatrecasas
      Pages 3-13

    3. Weak-Affinity Chromatography

      • Sten Ohlson, David Zopf
      Pages 15-25

    5. 2-Fluoro-1-methylpyridinium (FMP) Salt-Activated Gels

      • Dyer Narinesingh, That T. Ngo
      Pages 49-68

    6. Slalom Chromatography

      • Jun Hirabayashi, Ken-ichi Kasai
      Pages 69-87

  3. Affinity Chromatography with Biological Ligands

    1. Front Matter

      Pages 89-89
      PDF

    2. Applications of Bacterial Immunoglobulin-Binding Proteins to the Purification of Immunoglobulins

      • Michael D. P. Boyle, Ervin L. Faulmann, Dennis W. Metzger
      Pages 91-112

    3. Affinity Chromatography of Oligosaccharides and Glycopeptides with Immobilized Lectins

      • Tsutomu Tsuji, Kazuo Yamamoto, Toshiaki Osawa
      Pages 113-126

    4. Selective Isolation of C-Terminal Peptides by Affinity Chromatography

      • Shin-ichi Ishii, Takashi Kumazaki
      Pages 127-138

    5. Receptor-Affinity Chromatography (RAC)

      • Michele Nachman-Clewner, Cheryl Spence, Pascal Bailon
      Pages 139-149

    6. Immobilized Artificial Membrane Chromatography

      • Francisco M. Alvarez, Carey B. Bottom, Prashant Chikhale, Charles Pidgeon
      Pages 151-167

    9. Chromatography with Cyclodextrin-Based Stationary Phases

      • Karen L. Williams, Apryll M. Stalcup
      Pages 189-202

  4. Immunoaffinity Separation

    1. Front Matter

      Pages 203-203
      PDF

    3. Immunoaffinity Purification of Organelles

      • Peter J. Richardson, J. Paul Luzio
      Pages 213-227

    4. Immunoaffinity Separation of Cells Using Monosized Magnetic Polymer Beads

      • John Ugelstad, Ørjan Olsvik, Ruth Schmid, Arvid Berge, Steinar Funderud, Kjell Nustad
      Pages 229-244
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About this book

Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica­ tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
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Keywords

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Editors and Affiliations

  • Department of Developmental and Cell Biology, University of California, Irvine, USA

    That T. Ngo

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Bibliographic Information

  • Book Title: Molecular Interactions in Bioseparations

  • Editors: That T. Ngo

  • DOI: https://doi.org/10.1007/978-1-4899-1872-7

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1993

  • Hardcover ISBN: 978-0-306-44435-7Published: 31 December 1993

  • Softcover ISBN: 978-1-4899-1874-1Published: 20 June 2013

  • eBook ISBN: 978-1-4899-1872-7Published: 11 November 2013

  • Edition Number: 1

  • Number of Pages: XVII, 570

  • Topics: Biochemistry, general, Biotechnology, Analytical Chemistry

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Buy it now

eBook USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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