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Fluorescence Microscopy and Fluorescent Probes

  • Book
  • © 1996

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Editors:
  1. Jan Slavík

    1. Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic

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Table of contents (46 chapters)

  1. Front Matter

    Pages i-xviii
    Download chapter PDF

  2. Fluorescence Microscopy and Fluorescent Probes

    1. Fluorescence Microscopy: State of the Art

      • Brian Herman
      Pages 1-14

    2. Fluorescence Lifetime-Resolved Imaging Microscopy: A General Description of Lifetime-Resolved Imaging Measurements

      • Robert M. Clegg, Peter C. Schneider
      Pages 15-33

    3. Confocal Fluorescence Lifetime Imaging

      • Hans C. Gerritsen
      Pages 35-46

    4. Multidimensional Fluorescence Microscopy: Optical Distortions in Quantitative Imaging of Biological Specimens

      • N. S. White, R. J. Errington, M. D. Fricker, J. L. Wood
      Pages 47-56

    5. Fluorescent Probes

      • Jan Slavík
      Pages 57-60

    6. Flow Cytometry versus Fluorescence Microscopy

      • José-Enrique O’Connor
      Pages 61-66

    7. Multichannel Fluorescence Microscopy and Digital Imaging - On the Exciting Developments in Fluorescence Microscopy

      • Heinz Gundlach
      Pages 67-70

    8. Fluorescence Lifetime Imaging and Spectroscopy in Photobiology and Photomedicine

      • Herbert Schneckenburger, Michael H. Gschwend, Karsten König, Reinhard Sailer, Wolfgang S. L. Strauß
      Pages 71-78

    9. A Versatile Time-Resolved Laser Scanning Confocal Microscope

      • I. V. Eigenbrot, B. Crystall, D. Phillips
      Pages 79-83

  3. Ion-Sensitive Fluorescent Probes

    1. Disappearance of Cytoplasmic Ca2+ Oscillations is a Sensitive Indicator of Photodamage in Pancreatic β-Cells

      • Anders Tengholm, Eva Grapengiesser
      Pages 85-89

    2. Distribution of Individual Cytoplasmic pH Values in a Cell Suspension

      • Petr Cimprich, Jan Slavík
      Pages 91-93

    3. The Effect of Lysosomal pH on Lactoferrin-Dependent Iron Uptake in Tritrichomonas foetus

      • Martin Gregor, Jan Tachezy, Jan Slavík
      Pages 95-99

    4. On the Protein-Error of the Calcium-Sensitive Fluorescent Indicator Fura-Red

      • N. Opitz, T. Porwol, E. Merten, H. Acker
      Pages 101-106

    5. Cytoplasmic Ion Imaging: Evidence for Intracellular Calibration Heterogeneities of Ion-Sensitive Fluoroprobes

      • N. Opitz, T. Porwol, E. Merten, H. Acker
      Pages 107-112

    6. The Effect of Protein Binding on the Calibration Curve of the pH Indicator BCECF

      • Jaromír Plášek, Jan Jaap ter Horst, Marcel Ameloot, Paul Steels
      Pages 113-118

    7. Artifacts in Fluorescence Ratio Imaging

      • Petr Cimprich, Jan Slavík
      Pages 119-123

    8. Use of Fluorescent Probes and CLSM for pH-Monitoring in the Whole Plant Tissue. pH-Changes in the Shoot Apex of Chenopodium rubrum Related to Organogenesis

      • Albrechtová Jolana T. P., Slavík Jan, Wagner Edgar
      Pages 125-132

    9. Spatial Resolution of Cortical Cerebral Blood Flow and Brain Intracellular pH as Measured by In Vivo Fluorescence Imaging

      • Robert E. Anderson, Frederic B. Meyer, T. M. Sundt Jr.
      Pages 133-138

  4. Membrane Potential-Sensitive Fluorescent Probes

    1. Is a Potential-Sensitive Probe diS-C3(3) a Nernstian Dye?: Time-Resolved Fluorescence Study with Liposomes as a Model System

      • Petr Heřman, Jaroslav Večeř, Aleš Holoubek
      Pages 139-143
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Keywords

About this book

Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Editors and Affiliations

  • Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic

    Jan Slavík

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