Genetic Engineering

1. Front Matter

   Pages i-xvi

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2. Cloning and Characterization of DNAs with Palindromic Sequences

   • David R. F. Leach

   Pages 1-11

3. DNA Isolation, Manipulation and Characterization from Old Tissues

   • Rob DeSalle, Elizabeth Bonwich

   Pages 13-32

4. Growth Factors and Neural Connectivity

   • Sarah McFarlane, Christine E. Holt

   Pages 33-47

5. Gene Identification by 3′ Terminal Exon Trapping

   • David B. Krizman

   Pages 49-56

6. Engineering Transgenes for USE in the Mammary Gland

   • Sinai Yarus, Darryl Hadsell, Jeffrey M. Rosen

   Pages 57-81

7. Problems That Can Limit the Expression of Foreign Genes in Plants: Lessons to be Learned From B.t. Toxin Genes

   • Scott H. Diehn, E. Jay De Rocher, Pamela J. Green

   Pages 83-99

8. Renaturation and Reconstitution of Functional Holoenzyme From Recombinant Subunits of Casein Kinase II Expressed as Inclusion Bodies in E. Coli

   • Wey-Jinq Lin, Rolf Jakobi, Jolinda A. Traugh

   Pages 101-110

9. Plant Acyl-ACP Thioesterases: Chain-Length Determining Enzymes in Plant Fatty Acid Biosynthesis

   • Toni Voelker

   Pages 111-133

10. Genetic Engineering of an Insect Parasite

    • Randy Gaugler, Sarwar Hashmi

    Pages 135-155

11. The Stop Signal Controls the Efficiency of Release Factor-Mediated Translational Termination

    • Warren P. Tate, Mark E. Dalphin, Herman J. Pel, Sally A. Mannering

    Pages 157-182

12. Mechanism of Replication and Copy Number Control of Plasmids in Gram-Positive Bacteria

    • Saleem A. Khan

    Pages 183-201

13. Pathways of Protein Remodeling by Escherichia Coli Molecular Chaperones

    • Marie Pak, Sue H. Wickner

    Pages 203-217

14. Pheromones and Pheromone Receptors as Mating-Type Determinants in Basidiomycetes

    • Lisa J. Vaillancourt, Carlene A. Raper

    Pages 219-247

15. Chemical Synthesis and Applications of Phosphopeptides

    • Kazuyasu Sakaguchi, Peter P. Roller, Ettore Appella

    Pages 249-278

16. Back Matter

    Pages 279-289

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Volume 18 explores the latest advances in recombinant DNA molecule techniques and how they are revolutionizing basic research in biology. Chapters discuss obtaining good expression of genetically engineered pest-resistant genes introduced in crop plants, cloning DNAs containing palindromes, and identifying genes by 3' terminal exon trapping and much more.

• Basidiomycetes
• DNA
• Escherichia coli
• Termination
• Translation
• biosynthesis
• cloning
• evolution
• genes
• genetic engineering
• Tree Biology

From a review of previous volume:
`A very important and useful book for all biotechnologists.'
Cellular and Molecular Biology

• Brookhaven National Laboratory, Upton, USA

  Jane K. Setlow

Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York.

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