Technologies for Detection of DNA Damage and Mutations

1. Front Matter

   Pages i-xxv

   PDF

2. Technologies for Detection of DNA Damage

   1. Front Matter

      Pages 1-1

      PDF

   2. Microgel Electrophoresis of DNA from Individual Cells

      • Narendra P. Singh

      Pages 3-24

   3. The Cytokinesis-Block Micronucleus Technique

      • Michael F. Fenech

      Pages 25-36

   4. Agarose Gel Electrophoresis for DNA Damage Analysis

      • Régen Drouin, Shuwei Gao, Gerald P. Holmquist

      Pages 37-43

   5. 32P-Postlabeling for Detection of DNA Adducts

      • R. C. Gupta

      Pages 45-61

   6. A Postlabeling Assay for Oxidative Damage

      • Michael Weinfeld, Michel Liuzzi, George D. D. Jones

      Pages 63-71

   7. Radioimmunoassay of DNA Damaged by Ultraviolet Light

      • David L. Mitchell

      Pages 73-85

   8. Monoclonal Antibody-Based Quantification and Repair Analysis of Specific Alkylation Products in DNA

      • Jürgen Thomale, Jörg Engelbergs, Frank Seiler, Manfred F. Rajewsky

      Pages 87-101

   9. Detection of Oxidative DNA Base Damages

      • Robert J. Melamede, Yoke Wah Kow, Ivan A. Bespalov, Susan S. Wallace

      Pages 103-115

   10. Strategies for Measuring Damage and Repair in Gene-Sized Specific DNA Sequences

       • Charles A. Smith, Philip C. Hanawalt

       Pages 117-129

   11. Methods to Measure the Repair of Genes

       • Vilhelm A. Bohr

       Pages 131-138

   12. Mapping and Quantification of Bulky-Chemical-Induced DNA Damage Using UvrABC Nucleases

       • Moon-shong Tang

       Pages 139-153

   13. The Use of DNA Glycosylases to Detect DNA Damage

       • Timothy R. O’Connor

       Pages 155-170

   14. PCR-Based Assays for the Detection and Quantitation of DNA Damage and Repair

       • F. Michael Yakes, Yiming Chen, Bennett Van Houten

       Pages 171-184

   15. DNA Damage Analysis Using an Automated DNA Sequencer

       • Gopaul Kotturi, Wolfgang C. Kusser, Barry W. Glickman

       Pages 185-197

   16. Ligation-Mediated PCR for Analysis of UV Damage

       • Silvia Tornaletti, Gerd P. Pfeifer

       Pages 199-209

   17. Ligation-Mediated PCR for Analysis of Oxidative DNA Damage

       • Régen Drouin, Henry Rodriguez, Gerald P. Holmquist, Steven A. Akman

       Pages 211-225

   18. Single-Strand Ligation PCR for Detection of DNA Adducts

       • Keith A. Grimaldi, Simon R. McAdam, John A. Hartley

       Pages 227-238

3. Technologies for Detection of Mutations

   1. Front Matter

      Pages 239-239

      PDF

Man-made carcinogens, natural genotoxic agents in the environment, as well as ionizing and ultraviolet radiation can damage DNA and are a constant threat to genome integrity. Throughout the evolution oflife, complex DNA repair systems have developed in all living organisms to cope with this damage. Unrepaired DNA lesions can promote genetic alterations (mutations) that may be linked to an altered phenotype, and, if growth-controlling genes are involved, these mutations can lead to cell transformation and the development of malignant tumors. Proto­ oncogenes and tumor suppressor genes may be critical targets for DNA damaging agents. In a number of animal model systems, correlations between exposure to a carcinogen, tumor develop­ ment, and genetic changes in tumor DNA have been established. To understand mutagenesis processes in more detail at the molecular level, we need to know the type and frequency of DNA adducts within cells, their distribution along genes and specific DNA sequences, as well as the rates at which they are repaired. We also need to know what types of mutations are produced and which gene positions are most prone to mutagenesis. This book provides a collection of techniques that are useful in mutagenesis research. The book is divided into three parts. In Part I, methods for DNA damage and repair analysis are provided.

• DNA
• Mammalia
• PCR
• Polymerasekettenreaktion
• cell biology
• genes
• genetics
• molecular biology
• mutagenesis
• polymer
• reaction
• transgen

• Department of Biology, Beckman Research Institute of the City of Hope, Duarte, USA

  Gerd P. Pfeifer

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