Troubleshooting LC Systems

1. Front Matter

   Pages i-viii

   PDF

2. Introduction

   1. Introduction

      • John W. Dolan, Lloyd R. Snyder

      Pages 1-9

3. General Considerations

   1. Logical Approaches to Troubleshooting

      • John W. Dolan, Lloyd R. Snyder

      Pages 11-77

   2. Separation Basics

      • John W. Dolan, Lloyd R. Snyder

      Pages 79-108

   3. Principles of Troubleshooting

      • John W. Dolan, Lloyd R. Snyder

      Pages 109-118

   4. Prevention of Problems

      • John W. Dolan, Lloyd R. Snyder

      Pages 119-137

4. Individual LC Modules

   1. Reservoirs and Degassing

      • John W. Dolan, Lloyd R. Snyder

      Pages 139-164

   2. Pumps

      • John W. Dolan, Lloyd R. Snyder

      Pages 165-200

   3. Tubing

      • John W. Dolan, Lloyd R. Snyder

      Pages 201-216

   4. Fittings

      • John W. Dolan, Lloyd R. Snyder

      Pages 217-234

   5. Injectors and Autosamplers

      • John W. Dolan, Lloyd R. Snyder

      Pages 235-290

   6. Columns

      • John W. Dolan, Lloyd R. Snyder

      Pages 291-326

   7. Detectors

      • John W. Dolan, Lloyd R. Snyder

      Pages 327-366

   8. Recorders and Data Systems

      • John W. Dolan, Lloyd R. Snyder

      Pages 367-383

5. Troubleshooting the Separation plus Other Problems

   1. Separation Problems

      • John W. Dolan, Lloyd R. Snyder

      Pages 385-423

   2. Separation Problems

      • John W. Dolan, Lloyd R. Snyder

      Pages 425-454

   3. Problems in Quantitation

      • John W. Dolan, Lloyd R. Snyder

      Pages 455-479

   4. Gradient Elution and Sample Pretreatment

      • John W. Dolan, Lloyd R. Snyder

      Pages 481-500

6. Back Matter

   Pages 501-515

   PDF

Over the last 15 years, high-performance liquid chromatogra­ phy (LC) has made the transition from an instrument used only by experts in research labs to a tool used for routine applications by relatively unskilled workers. With this transition have come in instrumentation and column technology. In major advances the past, the operator had to be a jack-of-all-trades, with a screw­ driver, soldering iron, and various wrenches as constant compan­ ions in the LC lab. Today, many instruments contain micropro­ cessors as powerful as those of mainframe computers of earlier days. With this technology has come a variety of self-diagnostic tools that allow the LC system to locate many of its own prob­ lems. Traditionally, well-honed LC troubleshooting skills have been a result of years of work at the bench. Today the LC system itself often can do a better job of troubleshooting than the operator can. Yet many of the problems of the past are still the major problems of today: air bubbles, check valves, detector lamps, and, of course, problems with the separation. An added pressure on the operator of today's LC system is that of productivity-the lab often cannot afford unnecessary downtime. This means that the operator has to be a troubleshooting expert, or has to have that expertise at his or her fingertips. The present book was written to provide this expertise in an easy-to-use format for users at all levels of experience.

• Chromat
• chromatography
• growth
• maintenance
• reactions
• system
• temperature

• LC Resources Inc., Walnut Creek, USA

  John W. Dolan, Lloyd R. Snyder

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