Human Tumor Cells in Vitro

1. Front Matter

   Pages i-xix

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2. Separation of Tumor Cells from Fibroblasts

   • John A. Sykes

   Pages 1-22

3. Development of Media for Isolation and Cultivation of Human Cancer Cells

   • Albert Leibovitz

   Pages 23-50

4. New Approaches to the Cultivation of Human Breast Carcinomas

   • Etienne Y. Lasfargues

   Pages 51-77

5. Old and New Problems in Human Tumor Cell Cultivation

   • Relda M. Cailleau

   Pages 79-114

6. New Human Tumor Cell Lines

   • Jørgen Fogh, Germain Trempe

   Pages 115-159

7. Human Brain Tumor Cells in Matrix Culture

   • Teddy Holmström

   Pages 161-174

8. Neoplastic Human Glia Cells in Culture

   • Jan Pontén

   Pages 175-206

9. Current Research with Organ Cultures of Human Tumors

   • Et. Wolff, Em. Wolff

   Pages 207-240

10. Metabolic Gradients in Tissue Culture Studies of Human Cancer Cells

    • Joseph Leighton, Ruy Tchao

    Pages 241-265

11. Human Lung Cancer in Tissue Culture

    • Russell P. Sherwin, Arnis Richters

    Pages 267-297

12. Cell Lines from Humans with Hematopoietic Malignancies

    • George E. Moore

    Pages 299-331

13. Cytological Characterization of Human Tumor Cells from Monolayer Cultures

    • Michael A. Bean, Steven I. Hajdu

    Pages 333-358

14. Chromosome Abnormalities in Human Tumor Cells in Culture

    • June L. Biedler

    Pages 359-394

15. Ultrastructural Characteristics of Human Tumor Cells in Vitro

    • Gabriel Seman, Leon Dmochowski

    Pages 395-486

16. The Strategy of a Search for Viral Involvement in Human Cancer

    • F. Kingsley Sanders

    Pages 487-515

17. Helper Activity for the Defective Friend Leukemia Virus in Human Malignant Cell Cultures

    • Audrey Fjelde

    Pages 517-549

18. Back Matter

    Pages 551-557

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The study of cultured human tumor cells is a most obvious approach in experimental human cancer research. For many techniques in virology, immunology, biochemistry, and biophysics, for example, large amounts of cells may be required and such quantities are usually provided only when the cultures develop into established cell lines; when this happens, thorough characterization also becomes possible. The development of cell lines, therefore, is of prime importance. Recent major advances in research with animal cell systems see m to be a prologue for present and future efforts directed toward work with human tumor cells in culture. Conceivably, the most significant results in cancer research may develop from work with such cells, and so the time seemed right to define the present state of our knowledge. This is the first book dedicated exclusively to the subject: human tumor cells in vitro. Although so me of the fundamental aspects in the cultivation of human tumor ceIls, and the extent to which they represent human cancer in vivo are still unclear, I asked a number of the leading investigators in this area of research to collect and evaluate previous and present contributions, and to offer their thoughts on the questions to which answers are not yet available. Many of the chapters are concerned with techniques of cultivation. Cultures from some types of tumors have grown weIl; in many cases they have given rise to established cell lines.

• biochemistry
• biophysics
• brain tumor
• cancer
• cancer research
• carcinoma
• cell
• cells
• chemistry
• development
• physics
• research
• tissue
• tumor
• virology

• Sloan-Kettering Institute for Cancer Research, Donald S. Walker Laboratory, Rye, USA

  Jørgen Fogh

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