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Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology

  • Book
  • © 1983

Overview

Editors:
  1. R. B. Cundall

    1. University of Salford, Salford, UK

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  2. R. E. Dale

    1. Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, UK

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: NATO Science Series A: (NSSA, volume 69)

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Table of contents (42 chapters)

  1. Front Matter

    Pages i-xiv
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  2. Old and New Developments in Fluorescence Spectroscopy

    1. Old and New Developments in Fluorescence Spectroscopy

      • G. Weber
      Pages 1-20

  3. Technique

    1. Front Matter

      Pages 21-21
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    2. Technique of Pulse Fluorometry

      • W. R. Ware
      Pages 23-57

    3. Phase and Modulation Fluorometry

      • F. W. J. Teale
      Pages 59-80

    4. Synchrotron Radiation as a Source to Study Time-Dependent Phenomena

      • I. H. Munro
      Pages 81-107

    5. Remarks on a Novel Method for Fluorescence Lifetime Measurements

      • E. Gratton, R. Lopez-Delgado
      Pages 109-111

  4. Data Analysis

    1. Front Matter

      Pages 113-113
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    2. Least Squares

      1. The Pile-Up Problem in Pulse Fluorometry
        • B. K. Selinger, C. M. Harris
        Pages 115-127
      2. Least Squares Methods of Analysis
        • B. K. Selinger, A. L. Hinde
        Pages 129-141
      3. Least Squares Methods of Analysis
        • B. K. Selinger, C. M. Harris, A. J. Kallir
        Pages 143-153
      4. A Critical Appraisal of Analytical Methods
        • B. K. Selinger, C. M. Harris
        Pages 155-168
      5. Estimators of Exponential Decay — A Postscript
        • P. Hall, B. K. Selinger
        Pages 169-172
      6. Beyond Chi-Square: Evaluation of Parametric Models Used in the Analysis of Data from Fluorescence Decay Experiments
        • R. Catterall, D. A. Duddell
        Pages 173-195

    3. Other Methods

      1. Fluorescence Decay Analysis by the Method of Moments
        • E. W. Small, I. Isenberg
        Pages 199-222
      2. Remarks on the Method of Moments for Fluorescence Decay Analysis
        • J. Eisenfeld
        Pages 223-231
      3. Systems Analysis Approaches
        • J. Eisenfeld
        Pages 233-238
      4. Fourier Transform Analysis
        • U. P. Wild
        Pages 239-257
      5. Analysis of Pulse Fluorometry Data by Laplace Transforms
        • A. Gafni
        Pages 259-270
      6. Modulating Functions — A Deconvolution Method
        • A. G. Szabo, L. Bramall
        Pages 271-283
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Keywords

About this book

At the time that the editors conceived the idea of trying to organize the meeting on which the contents of this volume are based and which became, in March 1980, a NATO Advanced Study Institute, the techniques of time-resolved fluorescence spectroscopy, in both the nanosecond and sub-nanosecond time-domains, might reasonably have been said to be coming of age, both in their execution and in the analysis and interpretation of the results obtained. These techniques, then as now, comprised mainly a number of pulse methods using laser, flash-lamp or, most recently, synchrotron radiation. In addition, significant developments in the more classical phase approach had also rendered that method popular, utilizing either modulation of an otherwise continuous source or, again recently, the ultra-rapid pulse rate attainable with a synchrotron source. In general terms, time-resolved fluorescence studies are capable, under appropriate conditions, of supplying direct kinetic information on both photophysics and various aspects of molecular, macromolecular and supramolecular structure and dynamics. The nanosecond and sub-nanosecond time-scales directly probed render these techniques particularly appropriate in studying relaxation and fluctuation processes in macromolecules, particularly biopolymers (e. g. proteins, nucleic acids), in supramolecular assemblies such as cell membranes, and in a variety of relatively simpler model systems.

Editors and Affiliations

  • University of Salford, Salford, UK

    R. B. Cundall

  • Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, UK

    R. E. Dale

Bibliographic Information

  • Book Title: Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology

  • Editors: R. B. Cundall, R. E. Dale

  • Series Title: NATO Science Series A:

  • DOI: https://doi.org/10.1007/978-1-4757-1634-4

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1983

  • Softcover ISBN: 978-1-4757-1636-8Published: 28 April 2013

  • eBook ISBN: 978-1-4757-1634-4Published: 11 November 2013

  • Edition Number: 1

  • Number of Pages: XIV, 785

  • Number of Illustrations: 1 b/w illustrations

  • Topics: Biological and Medical Physics, Biophysics

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