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Methods of Protein Separation

  • Book
  • © 1975
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Overview

Editors:
  1. Nicholas Catsimpoolas

    1. Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, USA

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Part of the book series: Biological Separations (BIOSEP)

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Table of contents (9 chapters)

  1. Front Matter

    Pages I-XIII
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  2. Sedimentation and Gel-Permeation Chromatography of Associating-Dissociating Macromolecules

    • John R. Cann
    Pages 1-25
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  3. Trans Electrophoresis

    • Nicholas Catsimpoolas
    Pages 27-67
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  4. Immunodiffusion

    • Alfred J. Crowle
    Pages 69-92
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  5. Isoelectric Focusing in Polyacrylamide Gel

    • James W. Drysdale
    Pages 93-126
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  6. Purification of Chemically Modified Proteins

    • Robert E. Feeney, David T. Osuga
    Pages 127-160
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  7. Chromatographic Peak Shape Analysis

    • Eli Grushka
    Pages 161-192
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  8. Sedimentation Equilibrium of Proteins in Density Gradients

    • James B. Ifft
    Pages 193-237
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  9. Hollow-Fiber Separation Devices and Processes

    • Burton A. Zabin
    Pages 239-254
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  10. Affinity Chromatography, Principles and Applications

    • Indu Parikh, Pedro Cuatrecasas
    Pages 255-276
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  11. Back Matter

    Pages 277-281
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Keywords

About this book

This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of proteins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area.

Editors and Affiliations

  • Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, USA

    Nicholas Catsimpoolas

Bibliographic Information

  • Book Title: Methods of Protein Separation

  • Editors: Nicholas Catsimpoolas

  • Series Title: Biological Separations

  • DOI: https://doi.org/10.1007/978-1-4757-1257-5

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1975

  • eBook ISBN: 978-1-4757-1257-5Published: 18 April 2013

  • Edition Number: 1

  • Number of Pages: XIII, 281

  • Number of Illustrations: 125 b/w illustrations

  • Topics: Biochemistry, general

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