Overview
- Editors:
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Lawrence J. Berliner
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Ohio State University, Columbus, USA
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Gareth R. Eaton
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Department of Chemistry and Biochemistry, University of Denver, Denver, USA
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Sandra S. Eaton
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Department of Chemistry and Biochemistry, University of Denver, Denver, USA
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Table of contents (13 chapters)
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Front Matter
Pages i-xviii
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- Sandra S. Eaton, Gareth R. Eaton
Pages 1-27
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- Sandra S. Eaton, Gareth R. Eaton
Pages 29-154
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- Eric J. Hustedt, Albert H. Beth
Pages 155-184
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- Hassane S. Mchaourab, Eduardo Perozo
Pages 185-247
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- Wenzhong Xiao, Yeon-Kyun Shin
Pages 249-276
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- Joseph C. McNulty, Glenn L. Millhauser
Pages 277-307
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- Sandra S. Eaton, Gareth R. Eaton
Pages 347-381
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- Petr. P. Borbat, Jack. H. Freed
Pages 383-459
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- G. Jeschke, M. Pannier, H. W. Spiess
Pages 493-512
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- K. V. Lakshmi, Gary W. Brudvig
Pages 513-567
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- Sergei A. Dzuba, Arnold J. Hoff
Pages 569-596
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Back Matter
Pages 597-614
About this book
Distance measurements in biological systems by EPR The foundation for understanding function and dynamics of biological systems is knowledge of their structure. Many experimental methodologies are used for determination of structure, each with special utility. Volumes in this series on Biological Magnetic Resonance emphasize the methods that involve magnetic resonance. This volume seeks to provide a critical evaluation of EPR methods for determining the distances between two unpaired electrons. The editors invited the authors to make this a very practical book, with specific numerical examples of how experimental data is worked up to produce a distance estimate, and realistic assessments of uncertainties and of the range of applicability, along with examples of the power of the technique to answer biological problems. The first chapter is an overview, by two of the editors, of EPR methods to determine distances, with a focus on the range of applicability. The next chapter, also by the Batons, reviews what is known about electron spin relaxation times that are needed in estimating distances between spins or in selecting appropriate temperatures for particular experiments. Albert Beth and Eric Hustedt describe the information about spin-spin interaction that one can obtain by simulating CW EPR line shapes of nitroxyl radicals. The information in fluid solution CW EPR spectra of dual-spin labeled proteins is illustrated by Hassane Mchaourab and Eduardo Perozo.
About the editors
Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR). He is the Series Editor for Biological Magnetic Resonance, which he launched in 1979.
