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Pharmaceutical Applications of Cell and Tissue Culture to Drug Transport

  • Book
  • © 1991

Overview

Editors:
  1. Glynn Wilson

    1. SmithKline Beecham Pharmaceuticals, King of Prussia, USA

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  2. S. S. Davis

    1. University of Nottingham, Nottingham, UK

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  3. L. Illum

    1. University of Nottingham, Nottingham, UK

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  4. Alain Zweibaum

    1. INSERM, Paris, France

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Part of the book series: NATO Science Series A: (NSSA, volume 218)

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Table of contents (32 chapters)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Pharmaceutical Applications of Cell Culture: An Overview

    • Ronald T. Borchardt, Ismael J. Hidalgo, Kathleen M. Hillgren, Ming Hu
    Pages 1-14

  3. Growth and Characterization of Cell and Tissue Cultures for the Study of Drug Transport

    • Glynn Wilson
    Pages 15-25

  4. Differentiation of Human Colon Cancer Cells

    • Alain Zweibaum
    Pages 27-37

  5. Criteria for Evaluating and Choosing a Relevant Cell Line

    • Jens Fogh
    Pages 39-49

  6. Use of Fabricated Living Tissue and Organ Equivalents as Defined Higher Order Systems for the Study of Pharmacologic Responses to Test Substances

    • Eugene Bell, Roger Gay, Mark Swiderek, Tom Class, Paul Kemp, Graham Green et al.
    Pages 51-65

  7. Intracellular Pathways; Exocytic and Endocytic Routes Relying upon Selective Vesicular Transport

    • Colin R. Hopkins
    Pages 67-76

  8. Carrier-Mediated Transport of Bile Acids and Amino Acids in Caco-2 Cells

    • Ismael J. Hidalgo, Ronald T. Borchardt
    Pages 77-91

  9. Passive Absorption of Drugs in Caco-2 Cells

    • Per Artursson, Johan Karlsson
    Pages 93-105

  10. Receptor-Mediated Transport of Cobalamin in Caco-2 Cells: Intracellular Localisation of Transcobalamin II

    • Ian F. Hassan, Ian J. Parsons, Martin W. Mackay
    Pages 107-120

  11. An in vitro Absorption Model System Based on Cell Monolayers (Intestine 407, MDBK)

    • H. Leuenberger, S. Buchmann, C. Reinke, B. Schmid
    Pages 121-139

  12. Transferrin Endocytosis and Fluid Phase Uptake in the Differentiable Intestinal Cell Line HT-29

    • Christian Huet, Odile Godefroy, Cristina Ibarra, Alice Dautry-Varsat, Daniel Louvard
    Pages 141-154

  13. Culture of Human Goblet Cells

    • Christian L. Laboisse
    Pages 155-161

  14. Endothelial Barriers

    • Kenneth L. Audus
    Pages 163-174

  15. Transport of Drugs Across the Blood-Brain Barrier: In Vitro and in Vivo Strategies

    • Joost B. M. M. van Bree, Albertus G. de Boer, Meindert Danhof, Douwe D. Breimer
    Pages 175-189

  16. Metabolism of Catecholamine Prodrugs by Cultured Bovine Brain Microvessel Endothelial Cells

    • Gerhard K. E. Scriba, Ronald T. Borchardt
    Pages 191-201

  17. Membrane Recycling, Adsorptive and Receptor-Mediated Endocytosis by Primary Bovine Cerebral Microvessel Endothelial Cell Monolayers in Vitro

    • Thomas J. Raub, Carolyn R. Newton
    Pages 203-216

  18. Lipoproteins and Reconstituted Blood-Brain Barrier

    • Stéphane Méresse, Marie-Pierre Dehouck, Pierre Delorme, Jean-Charles Fruchart, Roméo Cecchelli
    Pages 217-229

  19. Regulation of the Myocardial Blood Supply by Adenosine and Adenine Nucleotides: Consequences of Newly Detected Barrier Functions of the Coronary Endothelium

    • S. Nees, A. Dendorfer
    Pages 231-245

  20. Cultured Human Umbilical Vascular Endothelial Cells and Their Underlying Matrix as Models to Study Cell Adhesion

    • Eva Bastida
    Pages 247-248
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Keywords

About this book

In recent years there have been rapid advances in the growth and differentiation of mammalian cells in culture. This has led to increasing use of such in vitro systems in a wide variety of studies on fundamental aspects of cell structure and function, including normal growth and metabolism, mechanisms of differentiation and oncogenesis, mechanisms of protein and membrane synthesis and cell polarity. Recent advances in our ability to grow cells, including human cells, on permeable supports, to generate confluent cellular barriers with the morphological polarity corresponding to their in vivo counterparts has greatly facilitated such studies. In particular these new techniques have led to an increasing interest in the use of cell and tissue culture systems as a means for examining the transport of drugs across epithelial and endothelial barriers. An obvious question is whether these new in vitro methodologies will provide convenient systems that can substitute for and replace animal models. Various research groups both in academia and in the pharmaceutical industry have been investigating these types of methodologies in order to develop convenient well characterized systems that can be used to examine basic aspects of transcellular transport and to evaluate the permeability of drug molecules and delivery systems. Of particular note is use of confluent cell layers to study the transport of large molecules such as peptides and proteins produced through recombinant DNA technology.

Editors and Affiliations

  • SmithKline Beecham Pharmaceuticals, King of Prussia, USA

    Glynn Wilson

  • University of Nottingham, Nottingham, UK

    S. S. Davis, L. Illum

  • INSERM, Paris, France

    Alain Zweibaum

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