Mass Spectrometry in Biotechnological Process Analysis and Control

1. Front Matter

   Pages i-xi

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2. Introduction

   1. Introduction

      • Elmar Heinzle, Matthias Reuss

      Pages 1-6

3. Instrumentation and Gas Analysis

   1. Developments in Mass Spectrometric Instrumentation Relevant to Biotechnology

      • E. R. Schmid

      Pages 7-16

   2. The Application of Single Detector Magnetic Sector Mass Spectro- Meter Systems in Fermentation off-Gas and Liquid Analysis

      • M. J. Winter

      Pages 17-38

   3. A Micro-Processor Controlled Multiple Inlet System for Mass Spectrometers in Biotechnology

      • K. Schaefer, M. Schultis

      Pages 39-48

   4. The Application of a Quadrupole Mass Spectrometer to Biotechnology Process Control

      • Candace D. Bartman

      Pages 49-61

4. Membrane Inlet Systems

   1. Membrane Inlets for on-Line Liquid-Phase Mass Spectrometric Measurements in Bioreactors

      • Raimond Peter Cox

      Pages 63-74

   2. Optimisation of a MS-Membrane Probe for the Measurement of Acetoin and Butanediol

      • M. Griot, E. Heinzle, I. J. Dunn, J. R. Bourne

      Pages 75-90

   3. Methanogenesis in the Rumen: Mass Spectrometric Monitoring

      • D. Lloyd, A. G. Williams, K. Hillman, T. N. Whitmore

      Pages 91-103

   4. Gas Analysis and Membrane Probes

      • M. Reuss

      Pages 105-114

5. Applications and Computer Control

   1. Application of MS to Industrial Fermentation

      • S. Bohatka, J. Szilagyi, G. Langer

      Pages 115-123

   2. Characterization of Bioreactors by Mass Spectrometry Analysis

      • A. Luebbert, S. Froehlich, K. Schuegerl

      Pages 125-142

   3. Methanogenesis in Mesophilic and Thermophilic Anaerobic Digesters: Monitoring and Control Based on Dissolved Hydrogen

      • T. N. Whitmore, G. Jones, M. Lazzari, D. Lloyd

      Pages 143-162

   4. On-Line Mass Spectrometry in the Analysis of Eight Gases During the Microbial Oxidation of Gaseous Hydrocarbons

      • Anthony O’L. Richards, Stephen H. Stanley, Howard Dalton

      Pages 163-177

   5. The Use of Mass Spectrometry in the Screening of Microorganisms on Productivity

      • Marcel W. W. M. van Tilborg

      Pages 179-186

6. Pyrolysis-MS and HPLC-MS Interfacing

   1. Differentiation of Phage Sensitive and Phage Resistant Streptococcus Cremoris Strains by Pyrolysis Mass Spectrometry and Discriminant Analysis of the Cell Walls

      • Jaap J. Boon, B. Brandt-de Boer, G. B. Eijkel, Elly Vlegels, Lolke Sijtsma, Jan T. M. Wouters

      Pages 187-208

   2. Development of an On-Line Pyrolysis Mass Spectrometry System for the On-Line Analysis of Fermentations

      • E. P. Sandmeier, J. Keller, E. Heinzle, I. J. Dunn, J. R. Bourne

      Pages 209-215

   3. Developments in HPLC-MS Interfacing

      • E. P. Lankmayr

      Pages 217-223

   4. Mass Spectrometry for Control of Fermentation

      • C. L. Cooney

      Pages 225-230

7. Back Matter

   Pages 231-241

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This book is based on the contributions to the IFAC-Workshop "Mass Spectro­ metry in Biotechnological Process Analysis and Control" held in Graz, Austria from 23 to 24 October 1986. The idea to organize this workshop and further to prepare these proceedings was stimulated by the following facts. Biotechnological processes urgently need better on-line instrumentation. Mass spectrometry (MS) offers a great potential to especially analyse gases and volatile compounds. It is, however, considered that this potential by far is not exhausted. The main reason for this is that MS often still is considered to be a very expensive technique requiring the permanent attention of a MS expert. In addition methods have not yet been developed to a user friendly state. On-line MS-methods are available to a certain extent, but need further development. To stimulate such development an interdisciplinary effort is necessary. Needs of industrial and university users and experience of physicists and instrument manufacturers have to be brought into a hopefully fruitful discussion. An introductory article describes the bioprocess background including a brief summary of the state of the art in bioprocess sensor and parameter estimation development, and the potential MS offers for bioprocess monitoring. In the first chapter on "Instrumentation and Gas Analysis" a general overview on some developments in MS-instrumentation is given initially by Schmid. Then the presently available instrumentation for bioprocess monitoring is discussed by instrument manufacturers (Winter; Schaefer and Schultis; Bartman).

• development
• mass spectrometry
• spectrometry

• Chemical Engineering Laboratory, ETH, Zurich, Switzerland

  Elmar Heinzle

• Technical University of Berlin, Berlin, Federal Republic of Germany

  Matthias Reuss

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