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Terminal Transferase in Immunobiology and Leukemia

  • Book
  • © 1982

Overview

Editors:
  1. Umberto Bertazzoni

    1. CNR Institute of Biochemical and Evolutionary Genetics, Pavia, Italy

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  2. F. J. Bollum

    1. Uniformed Services University of the Health Sciences, Bethesda, USA

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 145)

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Table of contents (31 chapters)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Structures and Properties of Terminal Deoxynucleotidyl Transferase

    1. Terminal Transferase: Past to Present

      • F. J. Bollum
      Pages 1-11

    2. Purification of Terminal Deoxynucleotidyl Transferase from Pig Thymus: Identification of 42,000 and 57,000 Dalton Species

      • Tsuguhiro Kaneda, Saiko Kuroda, Osamu Koiwai, Shonen Yoshida
      Pages 13-18

    3. Purification of Terminal Deoxynucleotidyl Transferase of 60,000 Dalton from Mammalian Thymus and Thymona by Immunoadsorbent Column and Comparison of Peptide Structures

      • Hiromu Nakamura, Kazushi Tanabe, Shonen Yoshida, Mutsushi Matsuyama, Toshiteru Morita
      Pages 19-23

    4. Structure of Calf Thymus Terminal Deoxynucleotidyl Transferase

      • Lucy M. S. Chang, F. J. Bollum
      Pages 25-35

    5. Purification and Characterization of Multiple Forms of Terminal Transferase from Human Leukemic Cells

      • Martin R. Deibel Jr., Mary Sue Coleman, John J. Hutton
      Pages 37-60

    6. Purification and Properties of Chick Terminal Deoxynucleotidyl Transferase (TdT)

      • Claude Pénit, Maria-José Gelabert, Catherine Transy, Pierre Rouget
      Pages 61-73

    7. Restriction Analysis of Cloned Heteropolymeric DNA Synthesized in vitro by TdT

      • G. Damiani, E. Palla, V. Sgaramella, A. I. Scovassi, U. Bertazzoni
      Pages 75-80

    8. Terminal Deoxynucleotidyl Transferase Help DNA Polymerase α Bypass Thymine Dimer

      • Shonen Yoshida, Sigeo Masaki, Hiromu Nakamura, Toshiteru Morita
      Pages 81-85

    9. Inhibitors of DNA Polymerases: Their Selectivity and Mode of Action

      • Prakash Chandra, Ilhan Demirhan, Uwe Ebener
      Pages 87-100

  3. Development of Immunological Reagents for Detection of Terminal Deoxynucleotidyl Transferase

    1. Development of Antibodies and Immunological Methods for Terminal Transferase Assay

      • F. J. Bollum
      Pages 101-108

    2. Use of Immunofluorescence Tests for TdT in Human Hematologic Malignancy

      • Mary Sue Coleman, John J. Hutton
      Pages 109-114

  4. Use of Terminal Deoxynucleotidyl Transferase as a Marker for Studies on Differentiation of Lymphocytes

    1. Ontogeny of Terminal Deoxynucleotidyl Transferase Containing Lymphocytes in Rats and Mice

      • Irving Goldschneider
      Pages 115-132

    2. Effects of Biological Response Modifiers on the Growth and Differentiation of Terminal Deoxynucleotidyl Transferase Containing Lymphocytes

      • Irving Goldschneider
      Pages 133-155

    3. Murine Thymocyte Subpopulations: Two and Three Parameter Analysis with Various Markers

      • Roland Scollay
      Pages 157-159

    4. Terminal Transferase Positive Cells in the Human Bone Marrow and Thymus

      • G. Janossy, N. Tidman, K. F. Bradstock, A. V. Hoffbrand, F. J. Bollum
      Pages 161-176

    5. Disappearance of Nuclear TdT in RPMI-8402 Following TPA Treatment

      • N. Sacchi, U. Bertazzoni, D. Breviario, P. Plevani, G. Badaracco, E. Ginelli
      Pages 177-183

    6. Terminal Deoxynucleotidyl Transferase in Human Fetal Tissues

      • Claudio Casoli, Antonio Bonati, Riccardo Starcich
      Pages 185-191

    7. Effect of Nucleosides on Human T and B Lymphoblastoid Cell Lines

      • D. Breviario, P. Plevani, N. Sacchi, G. Badaracco, U. Bertazzoni, E. Ginelli
      Pages 193-199

    8. 20α-Hydroxysteroid Dehydrogenase (20αSDH) — A New Enzymatic Marker for Pre T and T Lymphocytes

      • Yacob Weinstein
      Pages 201-205
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Keywords

About this book

This book contains the proceedings of a conference devoted to the study of the structure and function of Terminal deoxynucleotidyl Transferase (TdT) and its utilization as biochemical marker in immuno­ biology and leukemia, held in Elba, Italy on May 28-31, 1981. The en­ zyme has been known to nucleic acid biochemists for more than 20 years and has proved to be an excellent tool for making deoxypolymers, label­ ing DNA fragments, and adding homopolymer tails to restriction endonu­ clease fragments from DNA. Since the discovery of its peculiar tissue distribution, normally restricted to the thymus and bone marrow, and of its abnormal occur­ rence in human leukemic lymphoblasts, TdT has become one of the most widely used markers in the study of lymphocyte differentiation and in the classification of hematopoietic neoplasia. The subject seemed most appropriate for a meeting where molecular and cellular biologists, immunologists and hematologists could convene for the first time to discuss both basic research and the clinical aspects of the problem. Among the goals achieved by this workshop was the sharing of in­ formation about the enzymology of TdT, biochemical and immunological methodology and the correlation of TdT with other markers in the diag­ nosis of leukemia. The remarkable accordance of results, presented here by five independent hematological institutions, from analysis of TdT in several thousands of leukemic patients, marks the importance of this enzyme as a diagnostic and prognostic tool in these diseases.

Editors and Affiliations

  • CNR Institute of Biochemical and Evolutionary Genetics, Pavia, Italy

    Umberto Bertazzoni

  • Uniformed Services University of the Health Sciences, Bethesda, USA

    F. J. Bollum

Bibliographic Information

  • Book Title: Terminal Transferase in Immunobiology and Leukemia

  • Editors: Umberto Bertazzoni, F. J. Bollum

  • Series Title: Advances in Experimental Medicine and Biology

  • DOI: https://doi.org/10.1007/978-1-4684-8929-3

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 1982

  • Softcover ISBN: 978-1-4684-8931-6Published: 15 June 2012

  • eBook ISBN: 978-1-4684-8929-3Published: 06 December 2012

  • Series ISSN: 0065-2598

  • Series E-ISSN: 2214-8019

  • Edition Number: 1

  • Number of Pages: XII, 393

  • Number of Illustrations: 102 b/w illustrations

  • Topics: Biochemistry, general

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