Advances in Tracer Methodology

1. Front Matter

   Pages i-ix

   PDF

2. Early History of Carbon-14

   1. Early History of Carbon-14

      • Martin D. Kamen

      Pages 1-19

3. Methods for Labeling Compounds

   1. Conditions for High Yield in the Labeling of Hydrocarbons by Exchange

      • B. E. Gordon, J. J. Madison

      Pages 21-38

   2. C14-Labeling Using Carbene Insertion—Application to Saturated Hydrocarbons

      • M. A. Muhs, E. L. Bastin, B. E. Gordon

      Pages 39-48

   3. Biosynthesis of Labeled Carbohydrates and Other Compounds of Biochemical Interest

      • S. Abraham

      Pages 49-59

   4. The Preparation of Labeled Albumin for Turnover Studies

      • Sheldon Margen, Harold Tarver

      Pages 61-72

   5. The Biosynthesis of C14- and H3-Labeled Insulin

      • G. Eric Bauer, Arnold W. Lindall, Arnold Lazarow

      Pages 73-81

   6. Purification of High Specific Activity Acetic-H3 Anhydride

      • Herbert H. Henderson, Frank Crowley, Leo E. Gaudette

      Pages 83-86

4. Special Analytical Techniques

   1. Automatic Counting of Radioactivity on Two-Dimensional Paper Chromatograms

      • V. Moses

      Pages 87-91

   2. A Convenient Method for the Determination of Metabolically Liberated C14O2

      • Eugene Roberts, Daisy G. Simonsen, Betty Sisken

      Pages 93-96

   3. Liquid Scintillation Counting of C14-Labeled Amino Acids on Paper, Using Trinitrobenzene-1-Sulfonic Acid, and an Improved Combustion Apparatus

      • Claude F. Baxter, Ilse Senoner

      Pages 97-105

   4. Zonal Scanning of Thin-Layer Chromatograms

      • Fred Snyder

      Pages 107-113

   5. Continuous Scintillation Counting of Amino Acid Analyzer Column Eluates

      • David H. Elwyn

      Pages 115-122

   6. Some Techniques of Radioactive Gas Chromatography for Lipid Research

      • H. J. Dutton

      Pages 123-134

   7. The Microchemical Identification of Steroids from Biological Media

      • David L. Berliner

      Pages 135-143

   8. Isotope Fractionation in the Absorption Chromatography of Doubly-Labeled Compounds

      • Peter D. Klein

      Pages 145-154

   9. Quench Correction by Automatic External Standardization

      • Ariel G. Schrodt, James A. Gibbs, Robert E. Cavanaugh

      Pages 155-162

5. Biochemical Applications

   1. Analytical Studies Using Low Levels of Carbon-14 and Tritium: A Method for Determining the Labeling Pattern in Myo-Inositol

      • Frank A. Loewus

      Pages 163-167

   2. Multiple Labeling to Determine Metabolic Pathways: Use of Labeling Ratios

      • J. T. Van Bruggen, Paul Russell

      Pages 169-177

   3. Diffusion of Radioactively Labeled Molecules in Heart Muscle

      • Ernest Page

      Pages 179-182

Advances in Tracer Methodology, Volume 2, records the pro­ ceedings of the 6th, 7th, and 8th Symposia on Advances in Tracer Methodology. These Symposia, which are part of a continuing series sponsored by the New England Nuclear Corp. and the Packard Instrument Company, Inc., are devoted to the entire isotope tracer field: preparation and analysis of labeled compounds, applications in the chemical, biochemical, and clinical fields, and health physics considerations associated with tracer work. The papers in this volume reflect certain trends which can be noted in the tracer field: increasing reliance on biochemical methods for labeling esoteric compounds, growing awareness of the problems of radiation decomposition, the use of advanced instrumentation for the analysis and detection of radioactive substrates, and the rapidly growing application of tracer compounds to clinical diagnosis. The three Symposia at which the papers were presented were held on the following dates: DATE LOCATION CHAIRMAN 11/16/62 New York City Daniel Steinberg, Chief Laboratory of Ce1lular PhYSiology and Metabolism, National Heart Institute, Bethesda, Maryland 3/8/63 Los Angeles Martin Kamen University of California San Diego, California 11/8/63 Chicago Joseph L. Rabinowitz Veterans Administration Hospital Philadelphia, Pennsylvania Once again, it is the editor's pleasure to acknowledge the help of his colleagues in planning and running the various Symposia, as well as the excellent cooperation of the chairmen, who acquitted themselves in their appointed tasks in a most efficient manner, With humor and understanding. S. R. Boston, Mass.

• Absorption
• Diffusion
• Sorption
• amines
• combustion
• decomposition
• diagnosis
• fields
• isotope
• metabolism
• paper
• physics
• physiology
• radiation
• tracer

• Boston, USA

  Seymour Rothchild

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