Overview
- Editors:
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Michael J. Morgan
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Department of Biochemistry, University of Leicester, Leicester, England, UK
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Table of contents (12 chapters)
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- Herman M. Kalckar, Donna B. Ullrey
Pages 1-27
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- Michael J. Morgan, Pelin Faik
Pages 29-75
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- Gagik Stepan-Sarkissian, Michael W. Fowler
Pages 151-181
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- Alan H. Fairlamb, Fred R. Opperdoes
Pages 183-224
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- William McCullough, Clive F. Roberts, Stephen A. Osmani, Michael C. Scrutton
Pages 287-355
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Back Matter
Pages 492-514
About this book
It is perhaps obvious to any student of Biology that the discovery of chemical processes in whole organisms has usually preceded the elucidation of the compo nent steps. However, it is perhaps less obvious that the unravelling of the se quences in which those chemical steps occur in living matter, of the precise mechanisms involved, and of the manner in which they are regulated, would have been achieved neither by the study of intact plants and animals nor even of extracts derived from them. Our ability to understand the nature and regulation of metabolism rests on two main premises: the postulate that life processes can indeed be validly investigated with individual cells and cell-free extracts, and the thesis that there is an essential "unity in biochemistry" (as Kluyver put it, 60 years ago) that enables events in one organism to be legitimately studied in another. Of particular utility in this latter respect has been the use of cultures of single-celled organisms, growing in defined media-especially prokaryotes, such as Escherichia coli, and eukaryotes, such as Neurospora and Sac charomyces sp. , to which both biochemical and genetical techniques could be applied. It was, of course, Pasteur's observations of bacterial fermentations that first overthrew the belief that oxygen was essential for all energy-yielding pro cesses: his recognition that "La fermentation . . . . . c' est La vie sans air" laid the foundations of our knowledge of glycolysis.
Editors and Affiliations
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Department of Biochemistry, University of Leicester, Leicester, England, UK
Michael J. Morgan
